FastQCFastQC Report
Wed 30 Apr 2025
2A1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8096133
Total Bases192.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG91609111.315167376820515No Hit
TGAAAATCTTTGCTCTGAAGTGGAA6255977.727108732032935No Hit
CGGACTGGCCGTGGTCGAGGC2775573.428266309360283No Hit
GTTGGATCCGGCCCGGACTGGCC2265302.798002453764038No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1676432.070655212803446No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1546641.9103441111948136No Hit
CGAGTGTGCTCTTAACTG1378441.7025906071454113No Hit
CTCGGGCTGAGACTTGAAGC1177561.454472153557754No Hit
CAGCTGTGACCCTCGGGT1058271.3071302064825268No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC979211.20947864863386No Hit
CTCGGGCTGAGACTTGAAGCGGTT947411.1702006377612622No Hit
CGTGGTGGACTGCTTGCAGTGCT931161.1501293271738495No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC855991.0572825322904158No Hit
CAGCTGTGACCCTCGGGTC800250.9884348490816541No Hit
CTCGGGCTGAGACTTGAAGCA597190.7376237519813472No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT535810.6618097800517853No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC501960.6199996961512366No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT481580.5948271847806847No Hit
CTTTCCGAGGTCGCGGTC450440.5563643779073294No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA398630.49237086396678514No Hit
CTCGGGCTGAGACTTGAAGCGG387930.4791546779184581No Hit
GAGTGTGCTCTTAACTGA382430.4723613112581031No Hit
TGAAAATCTTTACTCTGAAGTGGAA373640.46150427617728124No Hit
AGACTTGAAGCGGTTGGAT317630.392323100423375No Hit
GAGACCGAGTGTGCTCTTAACTG309620.38242948825074885No Hit
GATCCGGCCCGGACTGGCC300860.37160950789716524No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC290030.3582327513641389No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC277200.3423856796818926No Hit
GAGTGTGCTCTTAACTG276260.3412246315617592No Hit
GGACTGGCCGTGGTCGAGGC273780.3381614407767264No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT269080.3323562001760594No Hit
TCTCGGGCTGAGACTTGAAGCG259910.3210298052168856No Hit
CGGACTGGCCGTGGTCGAGGT259180.32012814018742036No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC228930.2827646235554678No Hit
GGAAGGAATCGGCCGTGGATTGGCC227300.28075131670885345No Hit
TTGGATCCGGCCCGGACTGGCC213800.26407668945161844No Hit
CGGTTAGGCGAACAAGAATAGAGGCC211110.26075411557591754No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC185450.2290599722114249No Hit
GAATCGGCCGTGGATTGGC183030.22607089088086868No Hit
GTTGGATCCGGCCCGGACTGGCT179700.22195781615741736No Hit
GAGACTTGAAGCGGTTGGA173420.21420102658886656No Hit
AGTGTGCTCTTAACTG167740.20718533156508173No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA167210.20653069805053847No Hit
GTTGGATCCGGCCCGGACTGGC163980.20254113908454813No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT163620.20209648235768857No Hit
GAGACTTGAAGCGGTTGGAT162180.20031785545025013No Hit
GGATCCGGCCCGGACTGGCC158100.19527841254584133No Hit
CGGTTAGGCGAACAAGAATAGAGGC156640.19347508248691073No Hit
CTCGGGCTGAGACTTGAAGCGGTTG154000.19021426648994033No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA152970.18894205418809204No Hit
TTCTGCTGAACTACTTTGAGAACGAGT152610.1884973974612324No Hit
GAGTGTGCTCTTAACT147540.1822351485579597No Hit
GACCAAGATAGTCGAACTCGACCCTGTC138190.17068642523535618No Hit
CGAGTGTGCTCTTAACTGA137740.1701306043267817No Hit
GAGACCGAGTGTGCTCTTAACT135630.1675244218443546No Hit
CGACTGCTGCCGGTCGTTGGGGC131510.16243557263696137No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT129370.15979233542729598No Hit
AGACTTGAAGCGGTTGGA122190.15092390404159614No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT121710.15033102840578336No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCG115900.14315476289729925No Hit
TGAAAATCTTTGCTCTGAAGTGGA112790.13931342284026213No Hit
CTACCGATAGCCAACTCGAACGTTTCCG102760.1269247923669238No Hit
GACCAAGATAGTCGAACTCGACCCTGTT101440.12529438436843862No Hit
CTTTCCGAGGTCGCGGTCGGA98340.12146539588714761No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGA97960.12099603600879581No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGAC96860.11963736267672481No Hit
CTTTCCGAGGTCGCGGTCGGACCG92720.1145238103178394No Hit
CGGACTGGCCGTGGTCGAGGCGAGG92420.1141532630454564No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACA92200.1138815283790422No Hit
AGTGTGCTCTTAACT91080.11249815189547899No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGCGAG91070.1124858003197329No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC90440.11170765104772859No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG89890.1110283143816931No Hit
GACTGGCCGTGGTCGAGGC89610.11068247026080229No Hit
GGGTCTGTAGCTCAGTCGGTTAGAGCGCA89570.11063306395781788No Hit
CTACCGATAGCCAACTCGAACGTTTC88290.10905206226231709No Hit
GAGACCGAGTGTGCTCTTAACTGA88060.10876797602015678No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGC88040.10874327286866459No Hit
GACTTGAAGCGGTTGGAT84380.10422259614559198No Hit
AGACTTGAAGCGGTTGGATCCGGCCC82960.10246867238964577No Hit
CGTGGTGGACTGCTTGCAGT82100.10140643687548118No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA81860.10110999905757476No Hit
TGGAAATCTTTGCTCTGAAGTGGAA81720.10093707699712937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)