FastQCFastQC Report
Wed 30 Apr 2025
1H10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6232770
Total Bases148.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG4392117.046802625477917No Hit
TGAAAATCTTTGCTCTGAAGTGGAA3883836.231306465664544No Hit
CGGACTGGCCGTGGTCGAGGC2030083.2571071931099658No Hit
TGAAAATCTTTACTCTGAAGTGGAA1961123.1464661779593985No Hit
GTTGGATCCGGCCCGGACTGGCC1656042.656988786687139No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1237751.9858746592606498No Hit
CTCGGGCTGAGACTTGAAGCA854761.371396666329738No Hit
CTCGGGCTGAGACTTGAAGC834511.3389070990907734No Hit
CGTGGTGGACTGCTTGCAGTGCT832831.3362116683272447No Hit
TGAAAATCTTTGCTCTGAGGTGGAA732721.1755928744362458No Hit
CAGCTGTGACCCTCGGGT706281.1331719283721362No Hit
CTCGGGCTGAGACTTGAAGCGGTT587530.9426466883905551No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA469920.7539504907127971No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA440230.706315169659718No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC436300.7000097869807484No Hit
CGAGTGTGCTCTTAACTG404150.6484275851667878No Hit
CGGACTGGCCGTGGTCGAGGT388970.624072442910616No Hit
CAGCTGTGACCCTCGGGTC352850.5661206814947447No Hit
CTTTCCGAGGTCGCGGTC340050.5455840661535722No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT331840.5324117527198982No Hit
GTTGGATCCGGCCCGGACTGGCT323790.519496146977989No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC310200.49769203740872836No Hit
GAGTGTGCTCTTAACTG255760.41034724528580385No Hit
GAGTGTGCTCTTAACT238040.38191686842286815No Hit
AGTGTGCTCTTAACT234480.37620512228110453No Hit
GGACTGGCCGTGGTCGAGGC223070.35789865501213747No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT216350.34711693195802185No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT216020.34658747234375725No Hit
GATCCGGCCCGGACTGGCC210410.337586658901259No Hit
GAGTGTGCTCTTAACTGA187390.300652839748619No Hit
AGACTTGAAGCGGTTGGAT185740.298005541677296No Hit
CTCGGGCTGAGACTTGAAGCGG184080.29534219937523765No Hit
GGAAGGAATCGGCCGTGGATTGGCC181970.29195686669009124No Hit
TCTCGGGCTGAGACTTGAAGCG169060.2712437648108305No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC168150.269783739813919No Hit
GAGACCGAGTGTGCTCTTAACTG164110.26330187059686144No Hit
GTTGGATCCGGCCCGGACTGGCA159190.25540810907509826No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC159010.255119312921863No Hit
TTGTGTAACTCCCTAAGGAAGG148120.23764714565113104No Hit
GGATCCGGCCCGGACTGGCC144920.23251299181583793No Hit
CGACTGCTGCCGGTCGTTGGGGCG141660.22728257259613302No Hit
GAATCGGCCGTGGATTGGC140730.22579045913775098No Hit
GAGACCGAGTGTGCTCTTAACT137830.22113763222451654No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA134270.21542588608275295No Hit
AGTGTGCTCTTAACTG134270.21542588608275295No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT132240.21216890724348883No Hit
CGACTGCTGCCGGTCGTTGGGGC124670.20002342457687353No Hit
TTGGATCCGGCCCGGACTGGCC119710.19206548613216917No Hit
CGTGGTGGACTGCTTGCAGTGCC119030.19097447844216936No Hit
GTTGGATCCGGCCCGGACTGGC115270.18494184768569993No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA113990.1828881861515827No Hit
CTCGGGCTGAGACTTGAAGT110700.17760963423967194No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT110010.17650258231893684No Hit
ACCCGTAGATCCGAACTTGTGG108570.17419221309305494No Hit
GAGACTTGAAGCGGTTGGAT104400.16750176887643856No Hit
GAGACTTGAAGCGGTTGGA99300.15931921120144013No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA97870.1570248862062935No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGC96520.1548589150570292No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC94800.15209930737055916No Hit
TAAAGAGGACTGATAATCTCAGGTTATC94780.1520672189090886No Hit
TGAAAATCTTTGCTCTGAAGTGGAT94400.1514575381411475No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC92550.14848935545511868No Hit
CTTTCCGAGGTCGCGGTCGGA92270.1480401169945305No Hit
CGTGGTGGACTGCTTGCAGTGCTC92030.14765505545688354No Hit
TGGGGGTAGTCGTTCTATGT89420.14346751123497256No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC88530.14203957469953166No Hit
CAGCTGTGACCCTCGGGC88520.14202353046879637No Hit
ACCCGTAGATCCGAACTTGT84940.13627969586556218No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG84700.1358946343279152No Hit
TGAAAATCTTTGCTCTGAAGTGGA84310.13526890932923885No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCG79930.12824153626718138No Hit
CTCGGGCTGAGACTTGAAGCGGT78460.1258830343490936No Hit
GACTGGCCGTGGTCGAGGC77520.12437487665997622No Hit
CGGTTAGGCGAACAAGAATAGAGGCC74360.11930489974762426No Hit
GACTTGAAGCGGTTGGAT74070.11883961705630082No Hit
GTTGGATCCGGCCCGGACT72790.11678595552218356No Hit
CTACCGATAGCCAACTCGAACGTTTC72320.11603187667762488No Hit
AGACTTGAAGCGGTTGGA69740.1118924651479198No Hit
CGACCAAGATAGTCGAACTCGATCCTGTC67900.10894032669262624No Hit
CGGTTAGGCGAACAAGAATAGAGGC65990.10587587862218564No Hit
CGGACTGGCCGTGGTCGAGGA64900.1041270574720389No Hit
CTCGGGCTGAGACTTGAAGCGGTTG64500.10348528824262729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)