FastQCFastQC Report
Wed 30 Apr 2025
1G8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7021160
Total Bases177.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGGCTGAGACTTGAAGCGGTTGGA94665613.482900261495251No Hit
TCTCGGGCTGAGACTTGAAGCGGTT6110218.702564818349105No Hit
TCTCGGGCTGAGACTTGAAGCG3349674.770821345760529No Hit
TGAAAATCTTTGCTCTGAAGTGGAA2700023.845546889687744No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA2520513.589876886440417No Hit
CTCGGGCTGAGACTTGAAGCGGTT1985552.8279515065886547No Hit
CTCGGGCTGAGACTTGAAGCG1314481.8721692711745637No Hit
CAGCTGTGACCCTCGGGTC1058331.5073435158862638No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA1010721.4395342080226061No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA824381.1741364674783084No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC585170.8334377795122173No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC517860.7375704299574429No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC444780.6334850651459303No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCG377310.5373898330190452No Hit
CGGACTGGCCGTGGTCGAGGC302560.43092594386112837No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGG281570.40103059893237014No Hit
TCTCGGGCTGAGACTTGAAGCGGT280590.39963481817819274No Hit
GTTGGATCCGGCCCGGACTGGCC276710.394108665804511No Hit
TCTCGGGCTGAGACTTGAAGCGGTTG224320.31949136609904916No Hit
CTTTCCGAGGTCGCGGTC214650.3057187131471153No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT204750.29161847899777243No Hit
TCTCGGGCTGAGACTTGAAGCGGTC199830.28461108990537176No Hit
GTTGGATCCGGCCCGGACT191040.27209179110004617No Hit
CGGGCTGAGACTTGAAGCGGTTGGA187460.26699291854907165No Hit
TCGGGCTGAGACTTGAAGCGGTTGGA179780.25605455508776326No Hit
GCCGGTCTGCCGCGAGGTACGTTACT178010.25353360413378984No Hit
CGGGCTGAGACTTGAAGCGGTT175960.2506138586786229No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGAT168550.24006004705775116No Hit
TCTCGGGCTGAGACTTGAAGCGG165140.23520329973964416No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC155110.22091791100046146No Hit
AGCTGTGACCCTCGGGTC141810.20197517219376857No Hit
TCTCGGGCTGAGACTTGAAGCA139190.1982435950754576No Hit
GACCAAGATAGTCGAACTCGACCCTGTC135110.19243258948663755No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCA129480.1844139714804961No Hit
TCGGGCTGAGACTTGAAGCGGTT126410.18004147462812412No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGA122860.17498533005942038No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC117980.16803491161004735No Hit
CCTGACTGGCGCCGACACT102610.14614394202667363No Hit
TTTCCGAGGTCGCGGTCGGACCGGGA95120.1354761891197466No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG92820.13220037714565686No Hit
CTCGGGCTGAGACTTGAAGCGGTTG91720.13063368446239654No Hit
GAGACTTGAAGCGGTTGGA91630.13050550051558432No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGT86100.12262930911701202No Hit
CTCGGGCTGAGACTTGAAGCGG82900.11807165767480018No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC80960.11530858148795925No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGT77080.10978242911427742No Hit
CTCGGGCTGAGACTTGAAGCGGT76490.10894211212961961No Hit
GGATCCGGCCCGGACTGGCC74890.10666328640851369No Hit
TCTCGGGCTGAGACTTGAAGC74200.10568054281628676No Hit
CCTTTCCGAGGTCGCGGTCGGACCGGGAA71610.10199169368024658No Hit
CGGACTGGCCGTGGTCGAGGCGAGGG70920.10100895008801965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)