FastQCFastQC Report
Wed 30 Apr 2025
1G7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7468005
Total Bases197.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA192969125.839444403157202No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4429705.931570747475397No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1302561.7441873699870314No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1039781.3923129403368102No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT961361.2873049763624957No Hit
GTTGGATCCGGCCCGGACTGGCC459660.6155057475189157No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT453750.6075919874183265No Hit
TGAAAATCTTTGCTCTGAAGTGGA438990.5878276728523882No Hit
TGAAAATCTTTGCTCTGAAGTGGAT410650.5498791176492249No Hit
TTCTGCTGAACTACTTTGAGAACGAGT363680.48698414101222487No Hit
TTGTGTAACTCCCTAAGGAAGG359060.4807977498676019No Hit
CAGCTGTGACCCTCGGGTC357330.47848120080262396No Hit
ACCCGTAGATCCGAACTTGTGG352800.472415323771208No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA344370.4611271685008246No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC317230.42478546814042034No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT281100.37640574691634515No Hit
TCAAGTCTAGGCTGGTTAGTTT278980.3735669700274705No Hit
ACCCGTAGATCCGAACTTGT275830.3693489760652276No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT262090.3509504881156346No Hit
TGAAAATCTTTGCTCTGAAGTGG246770.3304363079564087No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT226590.303414365684008No Hit
TGATCATGGATTCTAAACTGGTAATGGCC216720.29019798460231344No Hit
CACCCCTCATCCGCTACTTTGACCTCATT196650.2633233373571657No Hit
TAAAGAGGACTGATAATCTCAGGTTATC172690.23123980233007344No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC149130.19969188558390094No Hit
TCGTCTGATTGGATACCCACGTACTTGATC146500.19617019538685365No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA145640.1950186160828762No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGC125140.16756817918573969No Hit
TGATCTTCTTGAATTTGCCCATTTACAATA124130.16621574302641737No Hit
TTACCGTGACCGATCCCGT124020.16606844799916443No Hit
TGAAAATCTTTGCTCTGAAGTGGAG122980.16467584046877312No Hit
GTTGGATCCGGCCCGGACTGGCT111190.14888849163866386No Hit
TTCGTCTGGTGAATGATAGAGCTTCATC106090.14205935855693722No Hit
TGAAAATCTTTGCTCTGAAGTGGGA105800.14167103530327044No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG103690.1388456488714188No Hit
CTCGGGCTGAGACTTGAAGCG96580.1293250339280705No Hit
TGAAAATCTTTGCTCTGAGGTGGAA93420.12509364950880456No Hit
TGAAAATCCTTGCTCTGAAGTGGAA91500.12252268176038983No Hit
CGAAAATCTTTGCTCTGAAGTGGAA87300.11689868981073258No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT83040.11119435511893738No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA82820.11089976506443154No Hit
CTGGATTTCAAAAAGAACCTAA81750.10946698616297124No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT81400.10899832016716647No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC75150.10062928452779557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)