FastQCFastQC Report
Wed 30 Apr 2025
1G6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12933159
Total Bases305.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG157026212.14136468901372No Hit
TGAAAATCTTTGCTCTGAAGTGGAA11833179.149481576774862No Hit
TGAAAATCTTTGCTCTGAGGTGGAA4863903.760798115912748No Hit
GTTGGATCCGGCCCGGACTGGCC4458113.4470387319911553No Hit
CTCGGGCTGAGACTTGAAGC2637902.0396408951594887No Hit
CTCGGGCTGAGACTTGAAGCGGTT2466291.9069509622513727No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2363601.827550407444925No Hit
CGGACTGGCCGTGGTCGAGGC1916081.4815251246814487No Hit
GGATCCGGCCCGGACTGGCC1808061.3980033803032965No Hit
CAGCTGTGACCCTCGGGTC1406171.0872595009463657No Hit
CGACTGCTGCCGGTCGTTGGGGCG1312581.014895123457463No Hit
TCTCGGGCTGAGACTTGAAGCG1159990.896911574349314No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC1061100.8204492034776655No Hit
GAGTGTGCTCTTAACTGA947900.732922250472603No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA869920.6726276233053348No Hit
CTTTCCGAGGTCGCGGTC801510.619732580415968No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC692130.5351592754716772No Hit
GATCCGGCCCGGACTGGCC654440.5060171300762637No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT604880.4676970259160968No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA599530.4635603722184193No Hit
CGAGTGTGCTCTTAACTG579070.44774057134842304No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC578330.4471683986874359No Hit
GGAAGGAATCGGCCGTGGATTGGCC564980.4368460946007081No Hit
ACCCGTAGATCCGAACTTGTGG554330.4286114475202849No Hit
GAGTGTGCTCTTAACTG536980.41519631823903197No Hit
CTCGGGCTGAGACTTGAAGCGG526720.4072632216150749No Hit
GAGTGTGCTCTTAACT495490.38311598890881954No Hit
CAGCTGTGACCCTCGGGT489410.3784148946131413No Hit
TTGTGTAACTCCCTAAGGAAGG434620.33605092151113275No Hit
TCTCGGGCTGAGACTTGAAGCGGTT421370.3258059380542681No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC419460.3243291140238823No Hit
TTGGATCCGGCCCGGACTGGCC407610.3151666193851015No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT377610.2919704304261627No Hit
AGGATGGACAGTGATTCAGAAA331370.2562173711774517No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC326160.25218896636158267No Hit
GTTGGATCCGGCCCGGACTGGC307430.23770681238821853No Hit
CTCGGGCTGAGACTTGAAGCGGT299590.2316448750069492No Hit
GGACTGGCCGTGGTCGAGGC294170.22745409686836757No Hit
CTTTCCGAGGTCGCGGTCGGA280850.21715498897059873No Hit
AGTGTGCTCTTAACT273970.21183532963601545No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA272600.21077603700689057No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG261840.20245633723361783No Hit
TGGATCCGGCCCGGACTGGCC260180.20117281477788992No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC259370.20054651767599857No Hit
TGAAAATCTTTGCTCTGAAGTGGA253750.1962010982776907No Hit
TTCTGCTGAACTACTTTGAGAACGAGT247480.1913530947852725No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA237360.1835282470431238No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT236430.1828091651853967No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT234850.18158749923355924No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC234150.18104625482451736No Hit
GGATCCGGCCCGGACTGGC231330.1788658130623771No Hit
AGTGTGCTCTTAACTG224230.17337604834209494No Hit
GTTGGATCCGGCCCGGACT222070.17170592273705132No Hit
CTCGGGCTGAGACTTGAAGCGGTTG202620.15666706022867266No Hit
TCCCTTTCCGAGGTCGCGGTC198330.1533500052075444No Hit
CGTGGTGGACTGCTTGCAGTGCT198200.15324948838872235No Hit
GTTCTATTTTGTTGGTTTCTGGAACC194750.1505819266584444No Hit
CCCTTTCCGAGGTCGCGGTC188020.14537824826865578No Hit
CGACCAAGATAGTCGAACTCGACCCTGT187120.14468236259988762No Hit
TCGGGCTGAGACTTGAAGCG185400.1433524477662418No Hit
GACTTGAAGCGGTTGGAT181980.14070808222492276No Hit
AGTGTGCTCTTAACTGA174210.1347002692845576No Hit
GAATCGGCCGTGGATTGGC172780.1335945842775149No Hit
GAGACTTGAAGCGGTTGGAT170730.13200951136532071No Hit
TCTCGGGCTGAGACTTGAAGC170180.1315842479010735No Hit
CTCGGGCTGAGACTTGAAGCA164550.1272310964397793No Hit
CTTTCCGAGGTCGCGGTCGGACCG162180.12539859751202317No Hit
GAGACCGAGTGTGCTCTTAACTG159470.12330320844273236No Hit
ACCCGTAGATCCGAACTTGT156590.12107637430267422No Hit
TGAAAATCTTTGCTCTGAAGTGG151770.11734951994327139No Hit
CGGACTGGCCGTGGTCGAGGCGAGG147190.11380823509554007No Hit
CGAGTGTGCTCTTAACTGA141230.10919992555569757No Hit
CTTTCCGAGGTCGCG138810.10732876631300983No Hit
CGACTGCTGCCGGTCGTTGGGGC136120.10524884136969166No Hit
CACCCCTCATCCGCTACTTTGACCTCATT132200.10221787267905699No Hit
TGAAAATCTTTGCTCTGAGGTGGA131470.10165343208105614No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC130210.10067919214478072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)