FastQCFastQC Report
Wed 30 Apr 2025
1G4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636017
Total Bases212.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA122730514.211470403543672No Hit
CTCGGGCTGAGACTTGAAGCG4393395.087287345543669No Hit
GTTGGATCCGGCCCGGACTGGCC3747404.339268901392853No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1663701.9264667959778219No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1258601.457384810613504No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC1068081.236773850723082No Hit
CTCGGGCTGAGACTTGAAGCGGTT1004181.1627814072158495No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT990221.1466165478831272No Hit
CTCGGGCTGAGACTTGAAGC964841.1172279998985644No Hit
CAGCTGTGACCCTCGGGTC855110.9901671106020287No Hit
CGGACTGGCCGTGGTCGAGGC767680.888928310354183No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA756610.8761099011268737No Hit
CGAGTGTGCTCTTAACTG629680.7291324229676713No Hit
CAGCTGTGACCCTCGGGT482100.5582434587611396No Hit
GTTGGATCCGGCCCGGACTGGCT418190.48423943584177753No Hit
CTCGGGCTGAGACTTGAAGCA412450.477592853279469No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA399780.46292173811144655No Hit
TTCCGAGGTCGCGGTCGGACCG378490.4382691696878318No Hit
CGGACTGGCCGTGGTCGAGGT377450.4370649108263683No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT375270.43454059898214653No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC341200.3950895418570853No Hit
GACCAAGATAGTCGAACTCGACCCTGTC322470.3734013029386116No Hit
GTTGGATCCGGCCCGGACTGGC321220.37195387642242944No Hit
TTCTGCTGAACTACTTTGAGAACGAGT309000.3578038348002326No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC297400.344371716730062No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA292710.3389409724413465No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC281990.3265278426385682No Hit
CTCGGGCTGAGACTTGAAGCGG280850.32520778965581004No Hit
AGACTTGAAGCGGTTGGAT270370.3130725657441388No Hit
CTTTCCGAGGTCGCGGTC261880.30324164484622945No Hit
TGAAAATCTTTGCTCTGAAGTGGA256280.2967571740537333No Hit
GGAAGGAATCGGCCGTGGATTGGCC230610.2670328231174163No Hit
CGTGGTGGACTGCTTGCAGTGCT230450.26684755252334497No Hit
GAGACCGAGTGTGCTCTTAACTG228690.2648095759885605No Hit
ACCCGTAGATCCGAACTTGTGG216470.25065953436636357No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC206360.23895274870348215No Hit
CGACTGCTGCCGGTCGTTGGGGCG202610.23461046915493566No Hit
GAGTGTGCTCTTAACTGA198630.23000186312741164No Hit
GGATCCGGCCCGGACTGGCC194420.22512693062091008No Hit
TTGGATCCGGCCCGGACTGGCC189690.21964986868367675No Hit
GATCCGGCCCGGACTGGCC186370.2158055038566969No Hit
GAATCGGCCGTGGATTGGC181680.2103747595679814No Hit
GAGTGTGCTCTTAACTG179910.20832520362106746No Hit
CTCGGGCTGAGACTTGAAGCGGT173470.2008680622096969No Hit
TCTCGGGCTGAGACTTGAAGCG160980.18640537646000466No Hit
TTCCGAGGTCGCGGTCGGACCGGGA160710.1860927323325093No Hit
TGAAAATCTTTGCTCTGAAGTGGAT160420.18575692938075505No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT158960.18406633520985427No Hit
GCACTGTGGTTCAGTGGTAGAATTCT155190.17970089683704882No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA151070.1749301790397124No Hit
GTTGGATCCGGCCCGGACTGGCA150990.17483754374267674No Hit
GAGACCGAGTGTGCTCTTAACT144110.16687090819761008No Hit
CGGACTGGCCGTGGTCGAGG142440.1649371463719907No Hit
CGACTGCTGCCGGTCGTTGGGGC140910.1631654963161837No Hit
GAGACTTGAAGCGGTTGGAT140850.163096019843407No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT140170.16230861981860387No Hit
GAGTGTGCTCTTAACT138230.16006221386548916No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG133080.1540988166188186No Hit
GTTGGATCCGGCCCGGACT132430.15334615483040387No Hit
TGAAAATCTTTGCTCTGAAGTGG132310.1532072018848504No Hit
CTCGGGCTGAGACTTGAAGCGGTTG119940.1388834690807116No Hit
TTGTGTAACTCCCTAAGGAAGG116440.13483067483540156No Hit
ACCCGTAGATCCGAACTTGT116000.13432118070170543No Hit
GAGACTTGAAGCGGTTGGA115910.13421696599254032No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA109090.12631980692025038No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCG107010.12391128919732326No Hit
AGACTTGAAGCGGTTGGATCCGGCCC101310.11731102428353256No Hit
GACTTGAAGCGGTTGGAT99260.11493724479699378No Hit
GGACTGGCCGTGGTCGAGGC98420.1139645741781194No Hit
CAGAGCTGTATTTCTGTAACATGTGATGC96230.1114286829217682No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG94770.10973808875086744No Hit
TTCCGAGGTCGCGGTCGGACC93360.10810539164061396No Hit
CTTTCCGAGGTCGCGGTCGGA90860.10521053860824961No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA89270.10336941207966588No Hit
AGTGTGCTCTTAACTG89020.10307992677642946No Hit
CGAGTGTGCTCTTAACTGA88630.10262832970338062No Hit
AGACTTGAAGCGGTTGGA88150.10207251792116667No Hit
GGATCCGGCCCGGACTGGC87840.1017135561451535No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC86890.10061351199285504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)