FastQCFastQC Report
Wed 30 Apr 2025
1G2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11999310
Total Bases283.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGTGGACTGCTTGCAGTGCT3524242.9370355462105735No Hit
CTCGGGCTGAGACTTGAAGCG2573622.1448066597162674No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1868851.5574645542118672No Hit
CTCGGGCTGAGACTTGAAGC1531131.2760150375313246No Hit
CTCGGGCTGAGACTTGAAGCGGTT1330681.1089637654165114No Hit
GAGACTTGAAGCGGTTGGA1294051.0784370101280825No Hit
CAGCTGTGACCCTCGGGTC1021210.8510572691263081No Hit
ACTGCTGCCGGTCGTTGGGGC1008330.840323318590819No Hit
CGACTGCTGCCGGTCGTTGGGGC871110.7259667430877276No Hit
CGACTGCTGCCGGTCGTTGGGGCG864210.7202164124437156No Hit
CGGTTAGGCGAACAAGAATAGAGGCC835900.6966233891782111No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT760360.6336697693450707No Hit
GAGACTTGAAGCGGTTGGAT758610.6322113521527488No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT703900.5866170638145027No Hit
CGAGTGTGCTCTTAACTG688500.57378299252207No Hit
GAATCGGCCGTGGATTGGC648510.5404560762243829No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT640800.534030706765639No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT578280.48192771084337355No Hit
GACCAAGATAGTCGAACTCGACCCTGTC515150.4293163523569272No Hit
CAGCTGTGACCCTCGGGT501820.4182073802576981No Hit
GGAAGGAATCGGCCGTGGATT495710.41311542080336283No Hit
CGGTTAGGCGAACAAGAATA493540.4113069834848837No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC445750.37147969341570475No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC440620.3672044475890697No Hit
CGGACTGGCCGTGGTCGAGGC421240.35105351891067066No Hit
GGAATCGGCCGTGGATTGGC415400.3461865723945794No Hit
GCCGGGAAGTTGTTCAAACTTGATCA369570.3079927095808009No Hit
CGTAGTTGGATTTCGGGATGGCCCG358610.29885885105060206No Hit
CGTGGTGGACTGCTTGCAGT335930.27995776423811036No Hit
CGGTTAGGCGAACAAGAATAGAGGC325410.27119059345912394No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC321130.26762372169733095No Hit
GACTGCTGCCGGTCGTTGGGGC320300.266932015257544No Hit
CGTGGTGGACTGCTTGCA307050.25588971365853536No Hit
GAGACCGAGTGTGCTCTTAACTG292890.24408903511951938No Hit
GGAAGGAATCGGCCGTGGAT279390.2328383882073219No Hit
GGAAGGAATCGGCCGTGGATTGGC278250.2318883335791808No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC272250.22688804606264862No Hit
GATAGGAATAGAACGATGTTTCCTTAGACGT270600.22551296699560222No Hit
CTAGTACTGTGAAACTGCGAATGGCT265190.2210043744181957No Hit
CTGCTGCCGGTCGTTGGGGC260240.21687913721705662No Hit
AGACTTGAAGCGGTTGGAT244940.20412840404989951No Hit
TGGGGGTAGTCGTTCTATGT243710.20310334510901043No Hit
GACTTGAAGCGGTTGGAT235610.19635295696169194No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC228000.1900109256282236No Hit
GAGACTTGAAGCGGTTGGATC217810.18151877066264643No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC212460.17706018096040524No Hit
TGGGGGTAGTCGTTCTATGTAGATCGGGT206420.1720265581937628No Hit
GAAAGACGAACTACTGCGAAAGCATT203690.16975142737374066No Hit
CTGCGTTCGCGCTCGTCCCCTT196730.1639510938545633No Hit
GTTCTATTTTGTTGGTTTCTGGAACC196190.16350106797807543No Hit
ACTGCTGCCGGTCGTTGGGGCG195360.16280936153828846No Hit
GAGTGTGCTCTTAACTG194360.16197598028553306No Hit
AACAGTAGTGGGATAGCA193020.16085924940684088No Hit
CTGACTGGCGCCGACACT187210.15601730432833222No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC174610.1455167005436146No Hit
GACTGCTGCCGGTCGTTGGGGCG172790.14399994666359983No Hit
CTCGGGCTGAGACTTGAAGCGG169280.1410747784664285No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT165870.13823294839453268No Hit
CTCGGGCTGAGACTTGAAGCGGTTG164980.1374912390795804No Hit
ACGATCTTCTGAGATTAAGCCTTTGTT162740.1356244650734084No Hit
AGTGTGCTTGGGATCTGCGA162130.13511610250922762No Hit
GAGACCGAGTGTGCTCTTAACT158900.13242428106282778No Hit
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTA157960.13164090268523773No Hit
CTATTTTGTTGGTTTCTGGAACC157940.13162423506018261No Hit
AGTCTTGTAATTGGAATGAGTACA157390.13116587537116717No Hit
GCCGGTCTGCCGCGAGGTA155940.1299574725546719No Hit
AGACTTGAAGCGGTTGGA154900.1290907560518063No Hit
ACGATCTTCTGAGATTAAGCCTTTGT151690.12641560223046158No Hit
GAGTGTGCTTGGGATCTGCGA151110.12593224110386347No Hit
ACGGTTAGGCGAACAAGAATA148340.1236237750337311No Hit
CGCGTAGAACAAAAGGGTAAAAGCT146050.12171533196492132No Hit
GAAGGAATCGGCCGTGGATT142650.11888183570555307No Hit
CGGTTAGGCGAACAAGAATAGA142190.11849848032928562No Hit
GACTGCTGCCGGTCGTTGGGGCGGT141920.11827346739104166No Hit
CTTTCTGGAGTTAAGGCT141900.11825679976598653No Hit
GAGTGTGCTCTTAACTGA141730.11811512495301812No Hit
GCTGCCGGTCGTTGGGGC141240.117706768139168No Hit
TCACTTTCTGGAGTTAAGGCT140040.11670671063586156No Hit
CTTGTGAATAGGCGGTTGCTCACA139650.11638169194728697No Hit
CCGATTGAATGGTTTAGTGAGGCCTC138080.11507328338046105No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATC135920.11327317987450945No Hit
CGTGGTGGACTGCTTGCAGTGCC135730.11311483743648593No Hit
TGCGTTCGCGCTCGTCCCCTT134800.11233979287142344No Hit
CGGTTAGGCGAACAAGAAT134590.11216478280834481No Hit
CGACCAAGATAGTCGAACTCGAC130540.10878958873468557No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC128340.10695614997862377No Hit
GAAGTAATGATTGAGAGAGACA127680.10640611835180523No Hit
GGAAGGAATCGGCCGTGGATTGGCC126730.10561440616168763No Hit
GAGTGTGCTCTTAACT125630.10469768678365672No Hit
CTCGGGCTGAGACTTGAAGCGGT122080.10173918333637517No Hit
AGGAATCGGCCGTGGATT121480.10123915458472195No Hit
AAGTAATGATTGAGAGAGACA120910.1007641272706514No Hit
TGAGACTTGAAGCGGTTGGA120570.10048077764471457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)