FastQCFastQC Report
Wed 30 Apr 2025
1G11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5837854
Total Bases145.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA3490085.978361226574012No Hit
CTCGGGCTGAGACTTGAAGCG3348855.736440137077769No Hit
CTCGGGCTGAGACTTGAAGCGGTT2572484.406550763345572No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2238963.8352449376089224No Hit
GTTGGATCCGGCCCGGACTGGCC1394642.388960052786521No Hit
CGGACTGGCCGTGGTCGAGGC1187352.0338809432370186No Hit
CAGCTGTGACCCTCGGGTC864241.4804070125768818No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC757911.2982681649798025No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA692991.1870629172980345No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT570780.9777222931577255No Hit
CTCGGGCTGAGACTTGAAGC510310.8741397095576561No Hit
CGACTGCTGCCGGTCGTTGGGGCG491730.8423129458187888No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT484950.8306990890830774No Hit
TCTCGGGCTGAGACTTGAAGCGGTT431940.7398951738087318No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC431090.7384391593212163No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC423980.7262600263727047No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC393500.6740490598086214No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA377990.647481077807016No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC352210.6033210148797828No Hit
TCTCGGGCTGAGACTTGAAGCG322930.5531655981804272No Hit
CTCGGGCTGAGACTTGAAGCGGT315080.539718876148667No Hit
GTTGGATCCGGCCCGGACTGGC281450.4821120911896734No Hit
CTCGGGCTGAGACTTGAAGCGG278750.47748710399403616No Hit
CTCGGGCTGAGACTTGAAGCGGTTG272510.4667982446974522No Hit
CTTTCCGAGGTCGCGGTC254930.4366844391791916No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA254440.4358450896510944No Hit
GGATCCGGCCCGGACTGGCC216800.3713693422274692No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA197110.33764119486372907No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT194040.3323824131264674No Hit
CTCGGGCTGAGACTTGAAGCA193610.3316458410916066No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG190440.32621576353228426No Hit
CAGCTGTGACCCTCGGGT186720.31984355895162847No Hit
CGTGGTGGACTGCTTGCAGTGCT175150.30002463233921234No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG166170.2846422675181668No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC161110.27597469892189835No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC160170.27436451819452834No Hit
GGAAGGAATCGGCCGTGGATTGGCC154310.2643265830217748No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC151210.2590164125378949No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC137990.2363711048614782No Hit
GGACTGGCCGTGGTCGAGGC135630.23232852346084706No Hit
TTGGATCCGGCCCGGACTGGCC127370.21817948855863814No Hit
GTTGGATCCGGCCCGGACT123590.21170450648474595No Hit
CGACCAAGATAGTCGAACTCGACCCTGT122670.21012858492178804No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA117060.20051888930418613No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT116640.19979944685153142No Hit
GAGACTTGAAGCGGTTGGAT111090.19029252872716584No Hit
CGACTGCTGCCGGTCGTTGGGGC102660.17585229092745383No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAA100880.1728032252947744No Hit
GGATCCGGCCCGGACTGGC100310.17182683910902877No Hit
CACCCCTCATCCGCTACTTTGACCTCATT97480.16697916734471263No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT92940.15920233702315953No Hit
GGTTGGATCCGGCCCGGACTGGC89220.1528301324425037No Hit
CTCGGGCTGAGACTTGAAGCGGTC89040.15252179996279455No Hit
GTTGGATCCGGCCCGGACTGGCT88780.15207643082543687No Hit
GATCCGGCCCGGACTGGCC87990.15072319383115781No Hit
TATTAATGCTGTAGGTGAAAGACA86750.14859912563760588No Hit
GCACTGTGGTTCAGTGGTAGAATTCT85760.14690329699920554No Hit
AGGATGGACAGTGATTCAGAAA85000.14560144875154468No Hit
CGGACTGGCCGTGGTCGAGGT82370.1410963686313498No Hit
CTTTCCGAGGTCGCGGTCGGA79450.1360945306271791No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA78310.13414175825568778No Hit
CGCCCCTCCCCGCCCTTTGACCTCATT78190.13393620326921502No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC75600.12949964147784443No Hit
CACCCCTCGCCTCTACTTGACCTCATT75490.12931121607357773No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA75200.12881445818960188No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG72560.12429224848720095No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA71980.12329873271924924No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC71640.12271632692424306No Hit
TGGATCCGGCCCGGACTGGCC70860.12138021951217007No Hit
GAGTGTGCTCTTAACTGA70190.12023253750436376No Hit
TGAAAATCTTTGCTCTGAAGTGGA67930.11636125192579327No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA66790.11440847955430197No Hit
TGATCTGCGCATAGGAATTGCCCTGGACTGC65400.11202746762765906No Hit
ACCCGTAGATCCGAACTTGTGG64770.11094830394867702No Hit
TTCTGCTGAACTACTTTGAGAACGAGT63810.10930386405689488No Hit
CGGACTGGCCGTGGTCGAGGCGAGG61420.10520989390964555No Hit
TGGGGGTAGTCGTTCTATGT61220.10486730226552429No Hit
CGAGTGTGCTCTTAACTG59870.10255480866770564No Hit
GGCCGTGATCGTATAGTGGTTAGTACATT59790.10241777201005713No Hit
CGGACTGGCCGTGGTCGAGGCGA59270.10152703373534179No Hit
TGAAAATCTTTGCTCTGAGGTGGA58660.10048212922077189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)