FastQCFastQC Report
Wed 30 Apr 2025
1F5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11044850
Total Bases291.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA240476921.772762871383495No Hit
TGAAAATCTTTGCTCTGAGGTGGAA8352627.562456710593625No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4837154.379552461101781No Hit
TTCTGCTGAACTACTTTGAGAACGAGT1318191.1934883678818635No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1246631.128697990466145No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1126851.0202492564407846No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA1025970.928912570111862No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT910390.8242665133523768No Hit
ACCCGTAGATCCGAACTTGTGG769460.6966685830952888No Hit
CACCCCTCATCCGCTACTTTGACCTCATT510270.4619981258233475No Hit
TGAAAATCTTTGCTCTGAAGTGGA510190.46192569387542615No Hit
TTGTGTAACTCCCTAAGGAAGG499110.4518938690883081No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT484420.4385935526512357No Hit
TGAAAATCTTTGCTCTGAAGTGGAT457450.41417493220822377No Hit
TGAAAATCTTTACTCTGAAGTGGAA444160.40214217485977627No Hit
TAAGTTGAAGATTGTAGCAGAAGCAAAAGC430110.3894213140060752No Hit
ACCCGTAGATCCGAACTTGT401920.36389810635726155No Hit
GTTGGATCCGGCCCGGACTGGCC394540.3572162591615097No Hit
TCAAGTCTAGGCTGGTTAGTTT376690.3410548807815407No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA321850.29140278048140084No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAA294380.26653146036388004No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA287170.26000353105746116No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC269180.24371539676862972No Hit
TGAAAATCTTTGCTCTGAAGTGG253980.2299533266635581No Hit
TATTAATGCTGTAGGTGAAAGACA250480.2267844289419956No Hit
AGGATGGACAGTGATTCAGAAA250330.2266486190396429No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA248390.22489214430254822No Hit
TACAACGGGGACTAGCTCCGATCTCTCAAGG238070.21554842302068383No Hit
TGAAAATCTTTGCTCTGAGGTGGA231440.2095456253366954No Hit
TACAACGGGGACTAGCTCCGATCTCTCAAG228200.20661213144587748No Hit
TCTCGGTGGGACCTCCATT221390.2004463618790658No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT214170.1939093785791568No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA196650.17804678198436377No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT190840.17278641176656995No Hit
TGATCATGGATTCTAAACTGGTAATGGCC190710.17266870985119762No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAA170880.15471464076017327No Hit
TGAAAATCTTTGCTCTGAGGTGGAT167280.15145520310370897No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA162000.14667469454089463No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT161820.14651172265807139No Hit
TGATCTGCGCATAGGAATTGCCCTGGACTGC160550.14536186548481872No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC144610.1309297998614739No Hit
TTACCGTGACCGATCCCGT144560.13088452989402302No Hit
TGAAAATCTTTGCTCTGAAGTGGAG136980.12402160282846758No Hit
TATTAATGCTGTAGGTGAAAGACC136030.12316147344690058No Hit
TGAAAATCTTTGCTCTGAGGTGG132580.12003784569278894No Hit
TAGGTGCTGCCCTGCAAATTCCCATATATC130670.11830853293616482No Hit
TGAAAATCTTTGCTCTGAAGTGGGA130490.1181455610533416No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA127700.11561949686958176No Hit
GTGGTCCGGACGGTCGAGGGTTATCAATCC126900.11489517739036745No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT126490.11452396365727013No Hit
TCGTCTGATTGGATACCCACGTACTTGATC123060.11141844389013884No Hit
TATCGTACTGCTCTCTATTGGACTGAGC123020.11138222791617813No Hit
TTCTGCTGAACTACTTTGAGAACGAGC120760.10933602538739774No Hit
TGAAAATCCTTGCTCTGAAGTGGAA118720.10748901071540129No Hit
CGAAAATCTTTGCTCTGAAGTGGAA110450.10000135809902352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)