FastQCFastQC Report
Wed 30 Apr 2025
1E4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11932270
Total Bases304.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA580642948.661562301221814No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2352141.9712426889435122No Hit
TGAAAATCTTTGCTCTGAAGTGGA1164740.9761260849779632No Hit
TCTCGGGCTGAGACTTGAAGCG1138530.9541604405532225No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1036840.8689377628900452No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT785530.6583240238445828No Hit
TTCTGCTGAACTACTTTGAGAACGAGT707300.5927623159717305No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA663590.5561305602370714No Hit
TCTCGGGCTGAGACTTGAAGCGGTT645800.5412214105111601No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT643740.5394949996941069No Hit
TGAAAATCTTTGCTCTGAAGTGG554280.46452183867780394No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA526760.44145833106357796No Hit
CTCGGGCTGAGACTTGAAGCG509450.42695145181931016No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA507250.42510771211177756No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA495260.41505933070572487No Hit
ACCCGTAGATCCGAACTTGTGG491000.41148918018113906No Hit
TTGTGTAACTCCCTAAGGAAGG472200.3957335863167695No Hit
TGAAAATCCTTGCTCTGAAGTGGAA314760.2637888683377094No Hit
TGAAAATCTTTGCTCTGAAGTGGAT312710.2620708381556904No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT310690.2603779498787741No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGC310570.2602773822583632No Hit
TGAAAATCTTTGCTCTGAAGTGGGA302670.25365668058131435No Hit
CTCGGGCTGAGACTTGAAGCGGTT300160.2515531411877203No Hit
CACCCCTCATCCGCTACTTTGACCTCATT290830.2437340087007753No Hit
ACCCGTAGATCCGAACTTGT285690.2394263622931764No Hit
TGAAAATCTTTGCTCTGAAGTGGAG259350.21735176961299066No Hit
TTACCGTGACCGATCCCGT258140.21633771277384772No Hit
AGGATGGACAGTGATTCAGAAA249430.2090381796590255No Hit
CAGCTGTGACCCTCGGGTC246190.206322853907932No Hit
TGAAAATCTTTGCTCTGAGGTGGAA241320.2022414846462576No Hit
GTTGGATCCGGCCCGGACTGGCC207610.17399036394583764No Hit
TGAAAACCTTTGCTCTGAAGTGGAA206260.17285897821621538No Hit
TGAAAATCTTTGCTCTGGAGTGGAA204920.1717359731216273No Hit
TGAAAATCTTTGCTCTGAAGTGGAAA203900.17088114834813492No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT195100.16350618951800455No Hit
TGAAAATCTTTGCCCTGAAGTGGAA188650.1581006799209203No Hit
CGAAAATCTTTGCTCTGAAGTGGAA185550.1555026830603062No Hit
TGGAAATCTTTGCTCTGAAGTGGAA175850.14737346707709428No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC175090.14673653881449214No Hit
GTTGGATCCGGCCCGGACT173780.1456386756250068No Hit
TCAAGTCTAGGCTGGTTAGTTT172820.14483413466171985No Hit
TTCGAAGACTCTAAACGGCGGAAGCCTATT172360.14444862545014486No Hit
TGAAAATCTTTGCTCCGAAGTGGAA170370.14278087907833129No Hit
TGAAGATCTTTGCTCTGAAGTGGAA163350.13689767328429545No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA153060.12827399983406343No Hit
TGAAAATCTCTGCTCTGAAGTGGAA151260.1267654855279004No Hit
TGAAAATCTTTGCTCTGAAGTGGTA147610.12370655374040312No Hit
AAGTGTGAAACTACTTGAACCGGTTAGC142200.11917263018687979No Hit
TGAAAATCTTCGCTCTGAAGTGGAA141300.11841837303379826No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA132420.11097636912339395No Hit
AAACTGACGAGCTTTTCGACTTCCAAATGA126350.10588932365761083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)