FastQCFastQC Report
Wed 30 Apr 2025
1C9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215942
Total Bases186.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA169621023.50642507935901No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2721823.771953821136589No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1586942.1992139072071257No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1192931.6531867911355165No Hit
CAGCTGTGACCCTCGGGTC552380.7654995009660553No Hit
TTCTGCTGAACTACTTTGAGAACGAGT534490.7407071730898058No Hit
GTTGGATCCGGCCCGGACTGGCC462870.6414547123577213No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT444800.6164129368002126No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT442150.6127405126038985No Hit
CTCGGGCTGAGACTTGAAGCGGTT429850.5956949210511947No Hit
GGATCCGGCCCGGACTGGCC396140.548978913633175No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT384740.5331805604867667No Hit
ACCCGTAGATCCGAACTTGTGG378580.5246439065059004No Hit
TGAAAATCTTTGCTCTGAAGTGGA349690.48460755366381825No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC343170.47557200432043384No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA327970.45450753345855605No Hit
TGAAAATCTTTACTCTGAAGTGGAA295960.41014742080798317No Hit
CGGACTGGCCGTGGTCGAGGC287220.39803535006240354No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT268250.3717463360985994No Hit
CTCGGGCTGAGACTTGAAGCG250210.346746135154634No Hit
CACCCCTCATCCGCTACTTTGACCTCATT239960.3325414755273809No Hit
TGAAAATCTTTGCTCTGAAGTGGAT236090.32717835038031073No Hit
CGGACTGGCCGTGGTCGAGG235460.32630528349590393No Hit
CGTGGTGGACTGCTTGCAGTGCT234780.32536292558892516No Hit
CGGACTGGCCGTGGTCGAGGT230620.3195979125109376No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC204380.2832339838651696No Hit
TGAAAATCTTTGCTCTGAAGTGG200070.27726109771946617No Hit
TCCCTTTCCGAGGTCGCGGTC182230.25253806086578856No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC181740.2518590088445833No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC172140.23855513251076574No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA166830.2311964259136229No Hit
ACCCGTAGATCCGAACTTGT165580.22946415034932377No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA163260.22624904690198452No Hit
GGATCCGGCCCGGACTGGC162320.22494637567763154No Hit
TCAAGTCTAGGCTGGTTAGTTT160390.22227174220635365No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA156960.21751837805791674No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA140150.19422273626922168No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC134040.1857553733109274No Hit
TTCCGAGGTCGCGGTCGGACCG133420.184896164631035No Hit
TTGTGTAACTCCCTAAGGAAGG132940.18423097081434414No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG130570.18094657634443292No Hit
CTTTCCGAGGTCGCGGTC127760.1770524208758884No Hit
TTACCGTGACCGATCCCGT123580.17125969138887204No Hit
GTTGGATCCGGCCCGGACTGGC113850.15777565839636737No Hit
TATTAATGCTGTAGGTGAAAGACA111000.1538260701097653No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAA103830.1438897374729453No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA102620.14221289472670373No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA102140.14154770091001284No Hit
TGAAAATCCTTGCTCTGAAGTGGAA99430.13779212748661226No Hit
CGACCAAGATAGTCGAACTCGACCCTGT97540.13517292683339197No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC96670.13396726304063972No Hit
TAAAGAGGACTGATAATCTCAGGTTATC95480.13231813670342693No Hit
CGCCCCTCCCCGCCCTTTGACCTCATT93960.13021168961723917No Hit
TGAAAATCTTTGCTCTGAAGTGGAG91390.1266501310570401No Hit
TGGATCCGGCCCGGACTGGCC89680.12428037808507883No Hit
TATTAATGCTGTAGGTGAAAGACC89520.12405864681284855No Hit
TGAAAATCTTTGCTCTGAAGTGGGA88660.12286684122461072No Hit
CAGCTGTGACCCTCGGGT88310.12238180406660697No Hit
AGGATGGACAGTGATTCAGAAA87310.12099598361516764No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT86200.11945772291407No Hit
GTAGTCGTGGCCGAGTGG85750.11883410371092229No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA85470.11844607398451927No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT83610.11586844794484213No Hit
CTCGGGCTGAGACTTGAAGC83480.11568829128615502No Hit
GTTGGATCCGGCCCGGACTGGCT79710.11046374818422876No Hit
TAGGTGCTGCCCTGCAAATTCCCATATATC78640.10898092030118868No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT76520.10604298094413729No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG75640.1048234589468707No Hit
TCGTCTGATTGGATACCCACGTACTTGATC73540.10191323599884812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)