FastQCFastQC Report
Wed 30 Apr 2025
1C2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9136369
Total Bases226.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA4612535.048537334689525No Hit
CAGCTGTGACCCTCGGGTC3001143.2848279223398267No Hit
CTCGGGCTGAGACTTGAAGCGGTT2956403.2358587968590147No Hit
CGTGGTGGACTGCTTGCAGTGCT2494212.7299794918528355No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA2360282.5833895281593815No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1444501.5810438479444078No Hit
CGGACTGGCCGTGGTCGAGGC1384131.51496726982021No Hit
CGGACTGGCCGTGGTCGAGG1383561.514343389589453No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT997221.0914839363427637No Hit
CTCGGGCTGAGACTTGAAGCG993131.0870073220554028No Hit
CGGACTGGCCGTGGTCGAGGT935641.024082980886608No Hit
GTTGGATCCGGCCCGGACTGGCC872640.9551277974871637No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC855120.9359516893417944No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC819660.8971397718283927No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC760190.8320482677527582No Hit
GGATCCGGCCCGGACTGGCC593900.6500394193798433No Hit
CTCGGGCTGAGACTTGAAGC457180.5003957261358424No Hit
CAGCTGTGACCCTCGGGT434430.4754952432415985No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA406390.4448047139952426No Hit
TTCCGAGGTCGCGGTCGGACCG389840.42669029676888054No Hit
TCCCTTTCCGAGGTCGCGGTC366520.40116593364387976No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC339590.3716903290574188No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG324570.35525053771361464No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT320610.3509162118999353No Hit
TTACCGTGACCGATCCCGT276450.30258191191708655No Hit
CCTGACTGGCGCCGACACT272100.297820720682363No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT264690.28971027768252355No Hit
CTCGGGCTGAGACTTGAAGCGGT242340.26524760547653015No Hit
CTTTCCGAGGTCGCGGTC241210.26401079028222263No Hit
GGACTGGCCGTGGTCGAGG229360.2510406486428033No Hit
GGATCCGGCCCGGACTGGC227280.248764033063901No Hit
GTTGGATCCGGCCCGGACTGGC219260.23998592876447963No Hit
CGGACTGGCCGTGGTCGAGGTGA217410.23796105433132134No Hit
CGACCAAGATAGTCGAACTCGACCCTGT216980.23749040784145212No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGGA216560.23703070661878914No Hit
CGGACTGGCCGTGGTCGAGGTGAGG210120.22998195453795703No Hit
CGTGGTGGACTGCTTGCAGTGCC207650.22727847353801056No Hit
GGAAGGAATCGGCCGTGGATTGGCC191680.20979888180961168No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT188570.2063949037084645No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA184820.2022904285061166No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCA178060.19489142787468414No Hit
GGTTGGATCCGGCCCGGACTGGC176080.19272426496784445No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA170220.1863103383849755No Hit
CCGAGGTCGCGGTCGGACCGGGAAG167060.182851633947797No Hit
GGACTGGCCGTGGTCGAGGC166550.18229342532027767No Hit
GACCGTGGTGGACTGCTTGCAGTGCT161390.17664566744184695No Hit
CTCGGGCTGAGACTTGAAGCGGTTG158400.17337303254717493No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT155130.16979393017072755No Hit
CGTGGTGGACTGCTTGCAGT154350.1689401993286392No Hit
TTGGATCCGGCCCGGACTGGCC151860.1662148277942802No Hit
CTCCCTTTCCGAGGTCGCGGTC145890.15968050327214237No Hit
TTCTGCTGAACTACTTTGAGAACGAGT144440.1580934395272345No Hit
CTCGGGCTGAGACTTGAAGCGGTC144000.15761184777015902No Hit
CGGACTGGCCGTGGTCGAGGTG143880.15748050456368387No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGG143610.15718498234911485No Hit
CTCGGGCTGAGACTTGAAGCGG134990.14775016201731783No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC131470.14389742796071392No Hit
TGAAAATCTTTGCTCTGAGGTGGAA129270.1414894691753365No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG125650.13752728244667No Hit
TCTCGGGCTGAGACTTGAAGCGGTT124920.13672827794061296No Hit
CGTGGTGGACTGCTTGCAGTGCTC122390.1339591253374289No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT119730.13104768426056346No Hit
CCTTTCCGAGGTCGCGGTCGGACCGGGAA119280.13055514723628173No Hit
CTTTCCGAGGTCGCGGTCGGA117270.12835514852782326No Hit
TGGATCCGGCCCGGACTGGCC116410.12741385554808482No Hit
GGACTGGCCGTGGTCGAGGT113890.12465564821210703No Hit
CTGACTGGCGCCGACACT112240.12284967912307394No Hit
CTCGTTGTGTGTGTGTCTCTCTGTCT112000.12258699271012367No Hit
CGGACTGGCCGTGGTCGAGGTGAGGG108730.11900789033367633No Hit
TTCCGAGGTCGCGGTCGGACC104720.11461883818396565No Hit
CTCCTGACTGGCGCCGACACT103700.11350242092892703No Hit
CTCCTGACTGGCGCCGACA102030.11167456130548144No Hit
CTCGTTGTGTGTGTGTCTCTCTGT101070.11062381565368036No Hit
TTCCGAGGTCGCGGTCGGACCGGGA100340.1098248111476233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA98980.10833625480757181No Hit
TGAAAATCTTTGCTCTGAAGTGGA97770.10701187747561422No Hit
GATCCGGCCCGGACTGGCC95900.1049651125080434No Hit
ATGTCGGTTCATCACTGAGGAGAGCGGCT95830.10488849563759957No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA94960.10393625739065486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)