FastQCFastQC Report
Mon 24 Feb 2025
POC-53-TP4_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-53-TP4_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19589811
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA352200.17978733944906358No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA218060.11131296774634528No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC206600.10546298787670796No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT203430.10384479972777684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)