FastQCFastQC Report
Mon 24 Feb 2025
POC-52-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-52-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15936004
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTT734380.46083070762281436No Hit
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA552180.3464984070034119No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA432160.2711846708873818No Hit
TCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTT425510.2670117301677384No Hit
CAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGCT397240.24927202578513408No Hit
TAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCGG385750.2420619372334495No Hit
CTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCG309670.19432098536119846No Hit
CATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAG268850.16870603195129721No Hit
TTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAA248160.1557228524792037No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC235730.14792290463782515No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT230030.14434609830670223No Hit
CTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAG218490.13710463426088498No Hit
ATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAGGAGGATGGCCTGGCT201380.1263679401686897No Hit
GAAGACCCCCGAAGAGCATCACCCCAACCCCAAAGCAACGCCGTTGCCTG201250.12628636388394482No Hit
AAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTT199790.12537019945527123No Hit
ACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGC187910.11791538204935188No Hit
ATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATAC183560.11518571405981072No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA177100.11113200021787142No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA171550.1076493203691465No Hit
TAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGT163200.10240961284899276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)