FastQCFastQC Report
Mon 24 Feb 2025
POC-52-TP1_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-52-TP1_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15936004
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT1156150.7254955508294301No Hit
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT848050.532159755983997No Hit
TATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTT838870.5263992152612412No Hit
TCGAAGTTTAACTTTGCCGAGTTCCTTAAAAAAATGTTTTTCTTTTTTTT557070.34956693032958575No Hit
CAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTT394400.2474898977183992No Hit
TTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTT338990.2127195751205886No Hit
CTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGT258070.16194147541629633No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG256080.16069273074981658No Hit
TTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCAC245240.1538905236218565No Hit
TTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAAC234870.14738324613874343No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT231180.14506773467175335No Hit
AAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAG223750.14040533624364052No Hit
TAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTA221270.1388491117346607No Hit
ATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAGGAGGATGGCCTGGCT204880.12856422475797571No Hit
GAAGACCCCCGAAGAGCATCACCCCAACCCCAAAGCAACGCCGTTGCCTG201540.12646834174991423No Hit
AGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAA200430.12577180578016925No Hit
ATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTTG180420.11321533302827987No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT163660.10269826739501321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)