FastQCFastQC Report
Mon 24 Feb 2025
POC-42-TP4_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-42-TP4_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868945
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTT588900.37110217471923934No Hit
TCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTT349930.22051245372644496No Hit
CTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCG288220.18162518050191742No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT280050.1764767601122822No Hit
TAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCGG276240.17407584436142415No Hit
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA268760.16936223548572385No Hit
TTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAA254270.1602311936930905No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC247130.15573183976628566No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA204540.12889325660905623No Hit
TTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACAT176820.11142517665793158No Hit
TTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACA173930.1096040095923201No Hit
ATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAA164460.1036363791039669No Hit
GTGACTCGGCAGTGTTCGGCTTATAAAACGTCAAGGTCTTGTAAAACAGC159360.10042255487053488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)