FastQCFastQC Report
Mon 24 Feb 2025
POC-42-TP4_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-42-TP4_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868945
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT571060.3598600915183713No Hit
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT567700.3577427484939925No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG533840.33640547623046146No Hit
TACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTA321450.20256545094837747No Hit
TTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAA228220.14381548363801122No Hit
CTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGT217120.13682068971818856No Hit
TACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCA212600.13397235922110762No Hit
ATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTC188530.11880436916253725No Hit
TATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTT188380.1187098449203775No Hit
TATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACA186620.11760076047903625No Hit
AGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAA184210.11608207098833603No Hit
CAGTGTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACA179760.11327785180426297No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT175030.11029718736815838No Hit
CAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTT169970.10710856959930228No Hit
AAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAG165810.1044870972834048No Hit
TTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCAC163640.10311964658016018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)