FastQCFastQC Report
Mon 24 Feb 2025
POC-259-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-259-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21755938
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT469210.21566985528272786No Hit
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT431720.1984377782286381No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG267930.12315258482534744No Hit
TATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACA222960.10248236596371989No Hit
TTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAA220530.10136542952089679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)