FastQCFastQC Report
Mon 24 Feb 2025
POC-255-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-255-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23909113
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA720750.3014540940937458No Hit
ACGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATG519510.2172853505690487No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC473550.19806255464182213No Hit
AAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTT448430.18755610047097943No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT439150.183674735235891No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA431660.180542038510588No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA421300.1762089626662436No Hit
TCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTT350040.14640442746663165No Hit
ACGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTG314990.1317447451940187No Hit
GAAATTTTTTTATTGATGAGACATTTTTGGTGTGTTTTTTGATCCGTTAT297830.12456756551361818No Hit
CAGTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTG296830.12414931494949227No Hit
CATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAG287120.12008810197182973No Hit
ACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTT283720.11866605005380167No Hit
TAATAAAGACAATTAAATGGCCGCGGTAACACTGACTGTGATAATGTAGC265740.11114590491081788No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA249600.1043953408058258No Hit
TGCTTGTGGTGGTCATAGATCTTGCTTAAACATTAGTTTATACTGATAAT241680.10108279633794864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)