FastQCFastQC Report
Mon 24 Feb 2025
POC-255-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-255-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23909113
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT774000.3237259366334502No Hit
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT645360.2699221840642938No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG537280.22471766309356606No Hit
TTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAA484950.20283061107285744No Hit
TATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACA394250.1648952849066379No Hit
GTTGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTT366570.15331810929163284No Hit
ATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACAC357220.14940746651705566No Hit
AATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTA356750.14921088875191646No Hit
TTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTTTT348740.14586070173326798No Hit
TATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTT337500.14115956539249283No Hit
CGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAA323300.13522040738190497No Hit
TGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTTAT311930.13046489846779344No Hit
TACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTA295480.12358467668792229No Hit
CAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCA291430.1218907619032124No Hit
GGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCA289190.12095388063957037No Hit
AAACCAACCCTTACGAGTGACTACACCGTAGGACGTTACAATTCAACATC286400.11978696156565909No Hit
AGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAA273050.11420331653457826No Hit
GTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTC267390.11183601834162564No Hit
AAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAG264960.1108196694707997No Hit
AATAACGGATCAAAAAACACACCAAAAATGTCTCATCAATAAAAAAATTT249400.10431169069300061No Hit
TTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAA247020.10331625435038097No Hit
CAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTT244560.10228735796263123No Hit
ACTCAAATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGT244130.1021075102200571No Hit
AAAGAATAGTTATATTAAGAGAACTATTGATTTTTGATCCTTTCGTACGA242850.10157214949797595No Hit
TACGAGTGACTACACCGTAGGACGTTACAATTCAACATCGAGGTCGCAAA240240.10048051552560733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)