FastQCFastQC Report
Mon 24 Feb 2025
POC-219-TP4_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP4_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19082344
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT425150.2227975766499126No Hit
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT373770.19587216329398524No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG269150.14104661356068207No Hit
TTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAA268080.14048588580103158No Hit
TATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACA223880.11732311292574958No Hit
GTTGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTT203900.10685270111470582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)