FastQCFastQC Report
Mon 24 Feb 2025
POC-219-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-219-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20948211
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAA3004381.4341940703194178No Hit
TCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACA2308781.102137075094384No Hit
CATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAG1436270.6856289541861117No Hit
AATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAAC1369690.6538458105085919No Hit
AAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTT1338800.6390999212295504No Hit
TTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGT1277750.609956621116715No Hit
CTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAG1200370.5730179059204625No Hit
GTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGA1088620.5196720617335772No Hit
ACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTT1074920.5131321237885182No Hit
TTTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTA985530.47046022211634203No Hit
TCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTTTTTGATTT973760.46484160389638995No Hit
ATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATAC926460.44226211011527433No Hit
TAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGT871780.41615964246302467No Hit
GACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCTTCAG807640.3855412760545519No Hit
TTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCTTCAGAAAAGT786510.37545449585169827No Hit
TGCTTGTGGTGGTCATAGATCTTGCTTAAACATTAGTTTATACTGATAAT763710.364570511534374No Hit
TAATAAAGACAATTAAATGGCCGCGGTAACACTGACTGTGATAATGTAGC748320.3572238221201801No Hit
TTTTACATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAG741440.35393953211565415No Hit
CAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGCT717500.3425113485824637No Hit
ATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCT680120.3246673427148504No Hit
TAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCGG673150.3213400896143351No Hit
GATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAATTTTTTATG666800.31830880450841365No Hit
CTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCG583450.27852020394486193No Hit
ACGAAAATGAGCTATGACGCATGTTTAACTTTGAAATTTTTTTATTGATG525490.2508519701276639No Hit
TAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGG513590.24517129410239374No Hit
AACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAA511610.2442261059906261No Hit
AAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTT485500.23176203447635696No Hit
ATTTTTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAA481620.22990984767147896No Hit
GAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAACTCGGCAAAGTTA451580.215569720965671No Hit
ATCAATTGTCAATTAATTGTTGACCGGTATGAATGGTATCCCGAAAGTTT423810.20231321901426333No Hit
TTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAA409290.1953818395279673No Hit
TTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACAT407180.1943745936108816No Hit
TTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACA401140.1914912925022571No Hit
TAACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGA372220.17768581765765104No Hit
TAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGG370060.17665470335390454No Hit
ATAAGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGG355230.1695753398703116No Hit
CCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAATTTTTTATGAAGAT355210.1695657925156473No Hit
TATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAA341010.1627871707039804No Hit
AAGAACTGTTTTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTT340420.16250552374138297No Hit
ACGCATGTTTAACTTTGAAATTTTTTTATTGATGAGACATTTTTGGTGTG339570.1620997611681494No Hit
ACAATAAATGAAAAGATTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGC332980.1589539078062561No Hit
TGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAA324840.15506813445787807No Hit
TATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAAAAAAAA319340.15244261192519018No Hit
GAGTTGTGCTTGTGGTGGTCATAGATCTTGCTTAAACATTAGTTTATACT319300.15242351721586153No Hit
GGTTATACATGCTAGTGTATGCGAACAGGTGAGGATAAAATAAAAAAATT319000.15228030689589675No Hit
AATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAATA307200.14664736764394823No Hit
GAAATTTTTTTATTGATGAGACATTTTTGGTGTGTTTTTTGATCCGTTAT305300.14574036895083783No Hit
TAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCT302890.14458991271378735No Hit
TGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATG289820.13835071644065453No Hit
TTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAATT288510.1377253647101416No Hit
AGTTGAAAGGAATTTGAATCTTTTATTTTTGAAGTAGCTTTAAAAAAGCG285830.13644601918512278No Hit
TTTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAA285760.13641260344379766No Hit
AGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTGTTTCTTCA285460.13626939312383288No Hit
GTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTAA284940.13602116190256056No Hit
CAGTTTTTTAAAAAGTAACGAAAATGAGCTATGACGCATGTTTAACTTTG284940.13602116190256056No Hit
CTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAATGGCCGCGGT278330.13286576118600293No Hit
AATTAATTGTTGACCGGTATGAATGGTATCCCGAAAGTTTTTCTGTCTTA263810.12593438169970697No Hit
TTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCG261230.12470277294800974No Hit
TTTCTAATTAAAGAAAGAAAAAAAAAAAGAAAAACATTTTTTTAAGGAAC259550.1239007951562069No Hit
AAAAATATTCAATGAAATTAAATCTGTAGTGAAGATGCTACATTTTAATT255050.121752640356735No Hit
ACACTGACTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACC255040.12174786667940284No Hit
TTTTTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAAA254680.12157601429544508No Hit
ATTTTTTTAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAAC254010.12125617791419038No Hit
TTTTTTGTTAAAAGAGAAAGCTCAAATCAGAAGCTATTGTCATTTTTTTT250930.11978588529588517No Hit
ATACAGTTTCTAAAAAAGAAATTTTTTATGAAGATTTTTTATAAAAATAT248740.11874044996014219No Hit
AATCTTTTATTTTTGAAGTAGCTTTAAAAAAGCGTACCTTTTGTATAATG245830.11735130985648368No Hit
AACATTAGTTTATACTGATAATGAAAGTACTGTAAAGGAAAGTTGAAAGA242800.11590488562483928No Hit
ATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGAATGGT240430.11477352409711743No Hit
TGGTTGAATCTTAATATGTTTGTTCCGCTTACTAATAAAGACAATTAAAT235400.11237236439904104No Hit
TTTATGAAGATTTTTTATAAAAATATATAATATCGGGAAAAGAAAAAAAA231620.11056791436748466No Hit
CTGTGATAATGTAGCGTAATCAATTGTCAATTAATTGTTGACCGGTATGA231360.11044379875684848No Hit
TGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTAATATGTT231020.11028149372755507No Hit
CGACTGTTTACCAAAAACATAGCTTTTTGATTTTTATAAAAGGTGAAACC230830.11019079385824404No Hit
AGTAATCTAAATATTATTTTTTTTTCTTAAGATTACCTTAGTCAAGGGTA230420.10999507308762549No Hit
AGCTTTTTGATTTTTATAAAAGGTGAAACCTGCCCGATGGTTGAATCTTA229240.10943177916243063No Hit
TCCGCTTACTAATAAAGACAATTAAATGGCCGCGGTAACACTGACTGTGA225430.10761300809887775No Hit
ATTAAATCTGTAGTGAAGATGCTACATTTTAATTGTTAGACGAGAAGTCC223740.10680625662974275No Hit
GTTTATACTGATAATGAAAGTACTGTAAAGGAAAGTTGAAAGAGAGTTGA219010.10454830725163118No Hit
TTTTCTTAAGATTACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAA217720.10393250287578255No Hit
CTAAGATAAACTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGC217460.1038083872651464No Hit
TTAAAAAAATATTCAATGAAATTAAATCTGTAGTGAAGATGCTACATTTT216820.10350287191588818No Hit
TAAGGAACTCGGCAAAGTTAAACTTCGACTGTTTACCAAAAACATAGCTT216800.10349332456122387No Hit
AATCAATTGTCAATTAATTGTTGACCGGTATGAATGGTATCCCGAAAGTT216770.1034790035292274No Hit
ACCTTAGTCAAGGGTAATACAGTTTCTAAAAAAGAAATTTTTTATGAAGA216110.10316394082530483No Hit
CTAAAATTAGACGATTTTTTTCGAAATGGCGTTCAGTTGCTTATTGGTTG210810.10063389183926016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)