FastQCFastQC Report
Mon 24 Feb 2025
POC-201-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOC-201-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15829437
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTT1116250.7051735320719239No Hit
AACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATT921520.5821558909517754No Hit
ACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAG730410.4614251283858043No Hit
TTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAA656120.41449357927259195No Hit
TATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACA564200.356424552559892No Hit
GTTGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTT547120.3456345288843817No Hit
AATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTA545390.34454162835987157No Hit
TTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTTTT539280.34068173113168837No Hit
ATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACAC528740.3340232504794706No Hit
TGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAACTTTTAT499630.3156334618849679No Hit
TATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTTT468680.29608128198115957No Hit
AAACCAACCCTTACGAGTGACTACACCGTAGGACGTTACAATTCAACATC465220.29389548093213924No Hit
GGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCA461270.29140013002357573No Hit
CAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCA459350.2901871999616916No Hit
CGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAA426930.2697063704792533No Hit
GTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTC400710.2531422943216489No Hit
TGTCACCACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAA392830.24816422719266645No Hit
TACGAGTGACTACACCGTAGGACGTTACAATTCAACATCGAGGTCGCAAA392230.24778518654832762No Hit
ACTCAAATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGT368520.23280676375287382No Hit
TACCATTCATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTA367490.2321560773134256No Hit
TTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACCAAAAA362570.22904794402984768No Hit
AGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGAA352830.22289485090341496No Hit
CAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTT350610.22149240051936148No Hit
AATAACGGATCAAAAAACACACCAAAAATGTCTCATCAATAAAAAAATTT350180.22122075472425204No Hit
AAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAG337520.21322299712870393No Hit
AAAGAATAGTTATATTAAGAGAACTATTGATTTTTGATCCTTTCGTACGA334510.21132147656293776No Hit
TGTCGTTGATTAACTCTCAAACATTATTGCGCTGTTATCCCCAGGGTAAC333900.2109361185745267No Hit
ATTTCAAAGTTAAACATGCGTCATAGCTCATTTTCGTTACTTTTTAAAAA317160.20036088459747495No Hit
ACTTTAATGGATGAACAAACCAACCCTTACGAGTGACTACACCGTAGGAC309600.1955849724788064No Hit
TTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAAACACACC309590.19557865513473413No Hit
ACATTTTTATTGTCTAAATTTAATTTTTTTTTAAACTAAAAAAAAAAAAA309450.19549021231772173No Hit
CTTTATTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGT300050.18955190888974763No Hit
TTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACG272700.1722739728519719No Hit
TAACAATTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATAT271550.17154747828365596No Hit
TACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCA270910.17114316826302794No Hit
CCCTTACGAGTGACTACACCGTAGGACGTTACAATTCAACATCGAGGTCG267280.16884997236477836No Hit
AAAACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTC262640.1659187247152252No Hit
TTCATTCAAAATAACGGATCAAAAAACACACCAAAAATGTCTCATCAATA257400.16260843642133324No Hit
TAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTAAGACAGA252410.1594560817292491No Hit
TTTCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTAT248420.1569354614443963No Hit
TCGTTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAA245960.15538139480260732No Hit
ATACCGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTC245340.1549897194701239No Hit
TAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAA241930.15283550514146524No Hit
TTAGTAAGCGGAACAAACATATTAAGATTCAACCATCGGGCAGGTTTCAC240290.15179946071360592No Hit
AATAAAAAAATTTCAAAGTTAAACATGCGTCATAGCTCATTTTCGTTACT237180.14983476670711662No Hit
TTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAACTT229680.14509675865288196No Hit
AAATCATGTATAACTTTAATGGATGAACAAACCAACCCTTACGAGTGACT229020.14467981394410934No Hit
ACAACATATTTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACC227770.1438901459350702No Hit
ACATCGAGGTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCG215130.13590502302766674No Hit
ACGCTTTTAACTCAAATCATGTATAACTTTAATGGATGAACAAACCAACC215030.13584184958694362No Hit
TAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAAAAAA212260.13409194527891294No Hit
GAGGTCGCAAACACTGTCGTTGATTAACTCTCAAACATTATTGCGCTGTT210510.1329864100662582No Hit
CAGTGTTACCGCGGCCATTTAATTGTCTTTATTAGTAAGCGGAACAAACA207600.13114806294121517No Hit
GATCCTTTCGTACGAAAAAACAACATATTTTTGTTTTCTAATTGTAGATA207370.13100276402755195No Hit
TTGACAATTGATTACGCTACATTATCACAGTCAGTGTTACCGCGGCCATT207010.13077533964094867No Hit
TTTTGTTTTCTAATTGTAGATAGAAACCAAGCTGTGTCACCACGCTTTTA201670.12740187790633362No Hit
TAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTTA199910.12629002534960657No Hit
TTTGCTCACACAATTCATGATTAAATCACTTGGTTTCGGGAAATTTGACT195720.12364305818330747No Hit
TTTTGCTCACACAATTCATGATTAAATCACTTGGTTTCGGGAAATTTGAC194120.12263228313173741No Hit
GGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATC188700.11920828264454382No Hit
ATTTATTCTGCTACGAAATATATAATTATTTTCAGAGCTTCTGTTTTATT182450.1152599425993483No Hit
ACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGATCAAAAA177480.11212022259540878No Hit
TTTTATAAAAATCAAAAAGCTATGTTTTTGGTAAACAGTCGAAGTTTAAC169070.10680733623059367No Hit
TTAAGATTCAACCATCGGGCAGGTTTCACCTTTTATAAAAATCAAAAAGC167880.10605557228598846No Hit
TTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAAAAAA167260.10566389695350505No Hit
CGGTCAACAATTAATTGACAATTGATTACGCTACATTATCACAGTCAGTG164090.10366129888258187No Hit
AGGGTAACTTTTATTTGTTTTCGTTATTTTTTTCATTCAAAATAACGGAT163640.1033770183993278No Hit
TTACTTTTTAAAAAACTGTCGCCCCAACAAAACTGTCTTACTTATAAAAA161920.10229043521888996No Hit
TTAAAATGTAGCATCTTCACTACAGATTTAATTTCATTGAATATTTTTTT161090.10176609566088801No Hit
GATTAGCCTTTCACCCCTAATCACAAATTTTCTGAGATTTTTGCCACAAT160900.10164606612351405No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)