FastQCFastQC Report
Wed 30 Apr 2025
2G2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2G2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19759778
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA9553584.834862011101541Illumina RNA PCR Primer (100% over 45bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG5673742.8713581701170936Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC3936751.992304771845109No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3702171.8735888631947182Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG3367681.7043106455953096Illumina Small RNA Adapter 1 (100% over 26bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC3226011.6326144959725761Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA3037571.537249052089553Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2612341.3220492659381091Illumina RNA PCR Primer (100% over 50bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT2399331.2142494718311108Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA2080091.0526889522746663Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1758970.8901770050250564No Hit
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG1519300.7688851565032765Illumina Small RNA Adapter 1 (100% over 26bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1515870.76714930704181Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1362170.6893650323399382Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT1037940.525279180768124No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC1001900.5070401094587196No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT992710.5023892474905336No Hit
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG976800.4943375375978414Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT967580.48967149327284953Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT960090.48588096485699384Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT938000.4747016894623006Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC930000.47065306098074583Illumina RNA PCR Primer (100% over 30bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT810990.41042465153201624Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA665120.3366029719564663Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG654050.33100068229511487Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA650230.32906746219517247Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA649620.3287587542734539No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG625300.31645092368952726No Hit
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG602800.30506415608515436Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG562610.28472485875094344No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC561860.2843452998307977Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG561260.28404165269468107Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG548050.2773563549145137No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT530310.26837852125666595Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG492170.24907668497085342Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG484440.24516469770055108Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC475250.240513835732365Illumina RNA PCR Primer (100% over 30bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA463060.2343447380835959Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT454270.22989630753948753Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT420200.21265421099366605Illumina RNA PCR Primer (100% over 31bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT417180.2111258537418791Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG398980.2019152239463419Illumina RNA PCR Primer (100% over 36bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG380910.19277038436363Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG376030.19030072098988157Illumina Small RNA Adapter 1 (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA343560.17386835014037103No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT317040.16044714672401683No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG311750.15776999114058873Illumina RNA PCR Primer (100% over 37bp)
GACCGCGACCTCGGAAAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG299320.15147943463737296Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT286150.14481437999961336Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA276250.13980420225368928Illumina DpnII expression Sequencing Primer (100% over 24bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT272460.13788616451055272No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT271270.13728393102392142Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA267640.13544686585041593Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT261720.13245088077406536Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT257360.130244378251618No Hit
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG253810.12844779936292808Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT241310.12212181736049868No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT228400.11558834314838963No Hit
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG226070.11440918010313678Illumina Small RNA Adapter 1 (100% over 26bp)
AGACCTACCTTCCACGGCACCTCGATCGTCGGACTGTAGAACTCTGAACG222570.11263790514245656Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC222320.11251138550240798No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221090.11188890887336893No Hit
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT219560.11111460867627157Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGGAGGATCGTCGGACTGTAGAACTCTGAACGT212820.10770363918056164Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATTC211080.10682306248582348No Hit
GACCGCGACCTCGGAAAGGGGATCGTCGGACTGTAGAACTCTGAACGTGT207180.1048493561010655Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGA205930.10421675790082258Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAGATCGTCGGACTGTAGAACTCTGAACGTG204590.10353861263016215Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC200210.10132198853651088Illumina RNA PCR Primer (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)