FastQCFastQC Report
Wed 30 Apr 2025
2G2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2G2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19759778
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGG8393304.247669179279241TruSeq Adapter, Index 15 (97% over 43bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA5348032.7065233222761913Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG3874611.9608570501146318No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3531251.7870899156862998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC3231021.63514994955915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT3108401.573094596508119Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2826671.4305170837445642Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2272551.150088831969671Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1964190.9940344471481409Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1720340.8706271902447487No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGGG1507810.7630703138466435TruSeq Adapter, Index 15 (97% over 39bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC1459530.7386368409604602Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1443220.7303826996436904Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1315640.6658171969340951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG1010420.5113518987915755No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG979140.4955217614286962No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA953670.48263194050054614No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA934780.4730721164984748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG914520.46281896486893725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC901560.4562601867288185Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG895570.4532287761532544Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA881490.44610319002571797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG766590.38795476345938706Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC641700.32475061207671463No Hit
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC627360.31749344552352765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA625620.3166128688287895Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA624550.3160713647693815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC593120.3001653156224731No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC580980.29402152190171366Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA553130.27992723400030106No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT550500.2785962473869899Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC543640.2751245484640566No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA539260.27290792437040534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC500580.2533328056620879Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA486380.24614649010732811Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGGG483860.24487117213563836TruSeq Adapter, Index 15 (97% over 43bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC465310.23548341484403318No Hit
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC457540.23155118443132305Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA448280.22686489696392337Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC437550.221434674013038Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT436010.2206553130303387Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT405200.20506303259075076TruSeq Adapter, Index 1 (100% over 35bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG402640.20376747147665322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAA380010.19231491365945508TruSeq Adapter, Index 15 (97% over 39bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC368590.1865354965020356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC356860.18059919499095586Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC337440.1707711493519816No Hit
CTTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC333720.1688885371080586No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA309030.1563934574568601No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAAT295120.14935390468455667TruSeq Adapter, Index 15 (97% over 41bp)
TCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCA291090.14731440808697346Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC273790.13855924899561117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA270830.13706125645743591Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG264310.13376162424496876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA255100.12910064070557878Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG251090.12707126567919944No Hit
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG250960.12700547546637417No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC246670.12483439844314041Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC243070.12301251562644074Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC233140.11798715552371083No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC222720.11271381692648572No Hit
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC217920.11028463983755284Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGTGCCGTGGAAGGTAGGTCTAGATCGGAAGAGCACACGTCTGAACTC214340.10847287859205705Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG214190.10839696680802789No Hit
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC212130.10735444497402755Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCC204410.10344751848932716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAG198890.10065396483705435Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)