FastQCFastQC Report
Wed 30 Apr 2025
2C3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23813975
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA18818287.90220028365697Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA13917095.844085248262837Illumina RNA PCR Primer (100% over 45bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG8487553.564104690628087Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5059832.1247313814682345No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG4678821.9647370924005758Illumina Small RNA Adapter 1 (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT4517631.8970499465124995Illumina RNA PCR Primer (100% over 50bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG3946301.6571361983877115Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC3464801.454943998219533Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2650391.1129557329257296No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT2489311.0453147784021777Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG2004010.8415268765504289No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT1569740.6591675686230459Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG1558210.6543258737778972Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT1459840.6130181962482114Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1456150.6114686859291656Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1436730.6033138104831302No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1413140.5934078624001243Illumina RNA PCR Primer (100% over 36bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC1239440.5204674986011365Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1150970.4833170438786468Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1014650.4260733455880423Illumina RNA PCR Primer (100% over 37bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGTCG1009650.42397373810966044No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA930720.39082933445592344No Hit
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG916990.3850638123202867Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT788740.3312088804997905Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGT715350.3003908419321008No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT708460.29749758282689054Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT693860.2913667289900153Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG685880.2880157554545178Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG684010.28723050225760294Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT666130.2797223059149092No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCA634250.2663352086327461No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA619150.2599943940480327Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT606620.25473277770720765Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG572150.24025808375124272Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG546830.2296256714807167Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC546530.22949969503201378Illumina RNA PCR Primer (100% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT545510.22907137510642384No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT533230.22391473913951787No Hit
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA518340.21766210806889652Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGATCGTCGGACTGTAGA516260.21678867135788965No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT511770.2149032238423027Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT498910.20950303340790438No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA480700.20185626297163745No Hit
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA459500.1929539272632981Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTC454650.19091730800926768No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG427480.17950804097174033Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA412720.1733099996955569Illumina DpnII expression Sequencing Primer (100% over 24bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA409100.17178988388120842Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403260.16933754234645831No Hit
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG386440.16227446278918156Illumina Small RNA Adapter 1 (100% over 26bp)
CCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT371130.15584546469037613Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC365230.15336792786588546Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT364720.15315376790309052Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG358030.15034449309701553Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG357460.15010513784447999Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT356200.14957603675992773No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT344240.14455377567163818No Hit
GGCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGT341380.14335280019400373No Hit
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG332660.13969108475170566Illumina Small RNA Adapter 1 (100% over 26bp)
GACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT330600.13882604647061234Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCATAGATCAGACAGTTCACTAGCAAAGTTGCCACGATCGTCGGACTG315710.13257341539999096No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT313460.1316285920347191Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG312940.1314102328569674No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT310780.1305032024263064Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC309810.1300958785755003Illumina RNA PCR Primer (100% over 32bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC303930.12762674018092318Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA302790.1271480296758521Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG302400.12698426029253831Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA301060.12642156548833197Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC297940.12511141042182164No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT278070.11676757030273191Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACG277640.11658700405959106Illumina Small RNA Adapter 1 (100% over 26bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC275130.11553300110544334Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTAACGTAGATC274950.11545741523622159No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC268620.11279931216859009No Hit
GGCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTGATCGTCGGACTGTA264010.11086347407352196No Hit
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTTGATCGTCGGACTGTAGAA257160.10798701182813872No Hit
CCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGA255480.10728154371540241Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA251940.105795021620708No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG247480.10392217174999133No Hit
CGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAAC244300.10258682139374044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)