FastQCFastQC Report
Wed 30 Apr 2025
2C3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23813975
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC17522627.358124798568907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGG10889944.572919892626073TruSeq Adapter, Index 19 (97% over 43bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA7746343.252854678817795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4833642.0297493383611935No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC4382591.8403437477363604Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA3667971.5402594484961036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT3253281.366122203454064Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2604931.0938661017322813No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG2353060.9881004746162705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA1933170.8117796377967138No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGGGGGGG1692530.710729729077149TruSeq Adapter, Index 19 (97% over 39bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG1475950.6197831315435579Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1463890.6147188783057008Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1365390.573356610981577No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1356810.5697536845486737Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1310890.5504708894672141Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA1298600.5453100542853514TruSeq Adapter, Index 19 (97% over 39bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT1189700.49958060340619326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTAGGGGG1185550.4978379291991362TruSeq Adapter, Index 19 (97% over 43bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1058590.44452469610806256Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGA1000250.4200264760503024No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAAT926780.3891748437629585TruSeq Adapter, Index 19 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC901440.3785340330625189No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA852580.35801666878377086Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTCCGAGG772870.32454472636340637No Hit
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC710350.29829123445371886Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTAGATCG710260.298253441519108No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC650680.27323451880670907Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA645500.27105932545910544Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC638320.268044289120149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA638290.26803169147527867No Hit
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC638100.26795190639110017Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG574840.2413876725746122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT568700.2388093545911592Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC550870.23132215432324926No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA534290.22435985592493485Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA533980.22422968026127513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG525560.22069394126768No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC516010.21668369098397056No Hit
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG502950.211199516250437Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGAAGAGCACAC493120.20707168794793815No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC479360.2012935681674311No Hit
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC478350.20086944745679797Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC477140.20036134244702952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC462190.1940835160866676No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCAGATCGG453890.19059816767255364No Hit
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC430380.18072581330920184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC388180.16300512619165847Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA386060.16211489262082454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT379920.15953657463737156Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC363040.15244829979035418Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG349250.14665758236497686No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCCAGATCG341790.14352496800723105No Hit
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG338270.14204684434245018Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC336960.1414967471831141Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA336800.1414295597438059Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT336050.1411146186220486Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACTGGCCGTGGTCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG335830.1410222358929998Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC321090.13483259304672993Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC308100.12937781281789368Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA305030.1280886538261672No Hit
GTGGCAACTTTGCTAGTGAACTGTCTGATCTATGATTAGATCGGAAGAGC301590.12664412388104046No Hit
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG300170.12604783535717998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC294260.12356609931773255Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG289870.12172264395171321Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT289190.1214370973346533No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT283420.11901415030460055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC283330.11897635736998968Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG281370.11815331123846395TruSeq Adapter, Index 19 (100% over 36bp)
TACGTTACTGGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGAT278670.11701952320013773No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA278280.11685575381682395Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGTG264280.11097685287735456TruSeq Adapter, Index 19 (97% over 43bp)
CGGACTGGCCGTGGTCGAGGTGAAGATCGGAAGAGCACACGTCTGAACTC256400.10766787149142466Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA253960.1066432630419743No Hit
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC253750.10655507952788226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCAC252850.10617715018177351No Hit
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT251920.10578662319079449Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCAAGATCGGAAGAGCACACG240930.10117168595331102No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC239500.1005711982144938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)