FastQCFastQC Report
Wed 30 Apr 2025
2A2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14820582
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA290029319.569359691812373Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC7912625.338940130691224No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG6394964.314918267042414Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC4253552.8700289907643306No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1481500.9996233616196719Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1314160.8867128160014229No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1188740.8020872594612007No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA1167750.7879245228021409Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACTC989690.6677807929540149Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG752100.5074699495606853Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT750980.5067142437456235No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA737800.49782120567195Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT723640.4882669250100975Illumina RNA PCR Primer (100% over 50bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC713530.4814453305544951No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG616980.41629944087216003Illumina RNA PCR Primer (100% over 36bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC600940.40547665402073957Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA511180.3449122308422166Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG495560.3343728336714442Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA466250.3145962823862113Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG417870.2819524901248817Illumina Small RNA Adapter 1 (100% over 26bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG411880.27791081348897095No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG400770.270414481698492No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA392910.265111046246362Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG392860.26507730937961815Illumina Small RNA Adapter 1 (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC375780.25355279569992595No Hit
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA369220.2491265187831355Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT368670.2487554132489534Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG348030.2348288346571005Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA335000.22603700718365852No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG326410.22024101347706856Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT325410.219566276142192Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACT319970.21589570504046332No Hit
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT316920.21383775616908973Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC306900.2070768880736263Illumina RNA PCR Primer (100% over 32bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA289420.19528247945998342No Hit
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT281160.18970914907390277Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC277400.1871721366947668Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA274380.18513442994343945No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG260940.17606596016269807Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT258470.17439935894555286Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACT246530.16634299516712636No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG243110.16403539348184842No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT235500.15890064236343754Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC232940.15717331478615348Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG230150.15529079762184778Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAAC215940.14570278009325138No Hit
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT215830.14562855898641497No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC204160.13775437428840515No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT200730.1354400252297784Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGAA198650.1340365715732351Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC194970.13155353818088925Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT194000.13089904296605895Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG192260.1297250000033737No Hit
AACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACT188980.12751186154497846No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT182170.12291690029446888No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT177550.11979961380733901Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGGCTGTGACCAACCGCCTATT168720.1138416831403787No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC163950.11062318605301734No Hit
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC157300.10613618277608801Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCCGGGAT156680.10571784562846452No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC156160.10536698221432869No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG154710.10438861307875764Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG152670.10301214891560938No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG152570.10294467518212173No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG151500.10222270623380378Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGTTCAGTTGAACTATGCAGTCATCACACACGATCGTCGGACTGTAGAAC151490.102215958860455No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)