FastQCFastQC Report
Wed 30 Apr 2025
2A2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14820582
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC286473819.329456832397Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8008645.4037284095860745No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT6320514.2646840724608515TruSeq Adapter, Index 9 (100% over 41bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG4212862.8425739286082017No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1482061.0000012145272028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1324590.8937503264041857No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1174800.7926814210130209No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1147080.7739777020902417Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGATCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT1005140.6782054847778582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA749020.5053917585692654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG732090.49396845548980467No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGG703330.47456300973875387TruSeq Adapter, Index 9 (97% over 45bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA695610.4693540375135066No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA612720.4134250598255858TruSeq Adapter, Index 9 (100% over 40bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT591500.3991071335795045Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA497710.33582351894142887Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA488130.32935953527331113Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC465430.3140429977716125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGGGGG416510.28103484734944956TruSeq Adapter, Index 9 (97% over 41bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC414810.2798877938801594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC391360.26406520337730327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC391310.2640314665105594No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA381410.25735156689528116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT374510.2526958792846327No Hit
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT366000.2469538645648329Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC360770.24342498830342832Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA345590.23318247556000163Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC333580.22507888016813374No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG332610.22442438495330347No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC324110.21868911760685242No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC323510.21828427520592647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA323320.21815607511229992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG315510.2128863765269137Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG304500.20545751846992247No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT296560.20010010403100228TruSeq Adapter, Index 9 (100% over 36bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC290590.196071922141789No Hit
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT278270.18775915817610941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC274380.18513442994343945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC256160.17284071570198795Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC251940.16999332414880874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGATCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC246280.16617431083340722No Hit
CCTGTGAATAGGCGGTTGGTCACAGCCCGATCAAGATAGTCGAACTCGAC243710.16444023588277437No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA240500.1622743290378205No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC232140.1566335249182522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA230660.15563491366263482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT228670.15429218636623043Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGATCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGT217290.1466136754953348No Hit
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG214620.14481212681121428No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG204010.13765316368817365No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG198900.13420525590695426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT196860.132828791743806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGCTGTTCTTCCTCGCCGAGACAGATCGGAAGAGCACACGTCTGAAC195270.13175595938135223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA194770.13141859071391393No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG193470.13054143217857436Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGATCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTC189040.12755234578507108No Hit
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC185500.12516377561960793No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA181540.12249181577349662No Hit
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG176390.11901691849888216Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT162930.10993495397144323No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT155290.10477996073298605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGA154260.10408498127806316No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA151310.10209450614017722No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA150820.10176388484608768No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACAAGATCGGAAGAGCACACGT150370.10146025304539322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)