FastQCFastQC Report
Wed 30 Apr 2025
2A1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757374
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG8793716.391997484403637Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA6045424.394312461084506Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6025464.37980387826921Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT4197253.050909279634326Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT3652572.654990698079444Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG2673901.9436122038987962Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2544641.84965531939453Illumina RNA PCR Primer (100% over 30bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG2168001.5758821414610085Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1613501.172825569763532Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1490311.0832808645021934Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1325330.9633597225749623Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1190270.8651869172125437No Hit
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT1130630.8218356206642343Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1010450.7344788329516956Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA948330.689324866795073Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC941510.6843675253722113Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG938490.6821723389943458Illumina Small RNA Adapter 1 (100% over 26bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC906310.6587812470606673Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC819130.595411595265201No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA730710.5311406086655782Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA688740.5006333330765014Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG650300.47269195414764476No Hit
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG570930.4149992578525523Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT516440.37539140827311956Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT481070.34968155986745725Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC451390.3281076752002235Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT451160.3279404921317106No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT430440.31287947830741536No Hit
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG427750.31092416328872063Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA423690.3079730186880142Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA422750.30728974875583087No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT383210.2785487986297385Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA373280.2713308513674194No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT372330.27064031260617033Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG367760.2673184577231091Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA360920.26234657864211586Illumina DpnII expression Sequencing Primer (100% over 25bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA304160.22108870486475107Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG298100.21668379445088864Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA287620.2090660615899517Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCTCTATTCTTGTTCGCCTAACCGTGATCGTCGGACTGTAGAACTCTG273910.1991004969407679Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT265730.19315459476496022No Hit
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT261070.18976732042030695Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT260350.18924396472757085Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC250100.18179341493514678No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT250040.1817498019607521Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG248740.18080485418220077Illumina RNA PCR Primer (100% over 37bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT241240.1753532323828661Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT238840.173608713407079Illumina RNA PCR Primer (100% over 31bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT218090.1585258930955864Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA215990.1569994389917727Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA212470.1544408111606183Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA212330.1543390475536974Illumina RNA PCR Primer (100% over 45bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG210350.15289981939867303Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT203790.14813146753152165Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGA200230.14554376438410413Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT196960.14316685727959422Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC193740.1408262943204132Illumina RNA PCR Primer (100% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTAACGTAGATC192830.14016483087542725No Hit
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT192490.13991769068719073Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT189680.13787514971970669Illumina RNA PCR Primer (100% over 50bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT188000.13665398643665572Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCAGTGATCGTCGGA186010.13520748945256558No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA177940.1293415443964815Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA176250.12811311228436473Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT171820.12489302100822439Illumina RNA PCR Primer (100% over 31bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG169310.12306854491271373Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA166630.12112049872308481Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA165690.12043722879090152Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT161220.11718806219849806No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT158280.11505102645315887Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT155660.11314659323792461Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC154870.11257235574172804Illumina RNA PCR Primer (100% over 32bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT154600.11237609735695198Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC154410.11223798960470217Illumina RNA PCR Primer (100% over 30bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT154180.11207080653618925No Hit
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT150920.10970116826074512Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA149980.10901789832856183Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA148350.10783307919083976Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG147300.10706985213893291Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA147130.10694628204481467Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCATAGATCAGACAGTTCACTAGCAAAGTTGCCACGATCGTCGGACTG146530.10651015230086788No Hit
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG146490.10648107698460477Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT141220.10265040406693894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)