FastQCFastQC Report
Wed 30 Apr 2025
2A1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757374
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA8398166.104478950706726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC5895754.285519896456984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5614494.081076810152868Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3416642.4834972139305074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3374752.4530480889739565Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA2543691.8489647806332807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC2115481.5377062512075343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1959541.4243561307557677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1578571.1474355498367639Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC1453891.0568077890446244Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1256350.913219339679215Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1182200.8593209721564595No Hit
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1074450.7809993389726847Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC986540.7170990626554167Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT914810.6649597517665798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT893970.6498115119934953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT815040.5924386441772972No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA782390.568705917277527Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT692020.5030175090100771Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA667320.4850635012176015Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA644910.46877405528118954No Hit
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC598650.4351484520228933Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA568220.4130294051757261Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG507820.3691256776184176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGTGCTCTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC502610.3653386176751464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG472670.34357574345220243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT469770.34146778302312636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG426640.31011732326241914No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC417620.30356083944508594Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA416640.30284849419663956Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTGTGCTCTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA387900.2819578794615891Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG376480.27365687666846883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC365780.2658792295680847No Hit
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC356300.2589883796137257Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC351900.2557900948247827Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC348190.2530933592413785Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGTGGACTGCTTGCAGTGTTAGATCGGAAGAGCACACGTCTGAACTC322530.23444154385858815Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT296460.21549170648410082Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA294970.21440865095329964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC284810.20702352062246765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT278130.20216794280652686Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGA273090.19850445295737398Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC259390.18854615713725598No Hit
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG255030.1853769476645761Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG249650.1814663176271867Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC238910.17365959521053945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT237640.17273645391918543TruSeq Adapter, Index 1 (100% over 41bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA236490.1719005385766208No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC236050.1715807100977265No Hit
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC234850.17070845060983295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT228730.16625992722157587TruSeq Adapter, Index 1 (100% over 35bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA225520.1639266330914606No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG214410.15585096399937953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC212150.15420820863051335Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA208310.151416978269254Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGG206310.1499632124560981TruSeq Adapter, Index 1 (97% over 45bp)
GAGTGTGCTCTTAAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA200210.14552922672597257Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC198800.14450432182769762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGAA198510.14429352578479004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC194880.14165494083391206Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC189340.13762800953147017Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC188020.13666852409478727No Hit
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC180730.13136954770583398Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGTTAGATCGGAAGAGCA174290.12668842178747194No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA171770.12485667686289549Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGGG168380.12239254380959622TruSeq Adapter, Index 1 (97% over 41bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGTTAGATCGGAAGAGCAC168290.1223271243480042No Hit
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT168200.12226170488641218Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT165100.12000836787602051TruSeq Adapter, Index 1 (100% over 35bp)
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA163440.11880174225110111Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT161010.11703541678811669Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTC159410.11587240413759195No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC159010.11558165097496079No Hit
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG151920.11042805116732307Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC151010.10976658772233712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC149810.10889432823444357TruSeq Adapter, Index 1 (100% over 36bp)
GAGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA147940.1075350571991428Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC147360.10711346511332759Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC147220.10701170150640667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG146240.10629935625796029Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA144600.10510726829117242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC144190.10480924629947548Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA144120.10475836449601501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGCAACTTTGCTAGTGAACTGTCTGATCTATGATTAGATCGGAAGAGC143800.10452576196591007No Hit
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA142160.10333367399912222Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG139660.10151646673267734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)