FastQCFastQC Report
Wed 30 Apr 2025
1H9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12904321
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG8289986.424189230878556Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG7740015.997998654869171Illumina Small RNA Adapter 1 (100% over 26bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC5863424.543764836600082Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA3999323.09920994680774Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT3904783.025947665127053Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT2496641.9347317848029355Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG2452461.9004951907194496Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA2076971.6095151383788424Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT1669391.2936674467412892Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1547411.1991409699123263Illumina RNA PCR Primer (100% over 30bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1262410.9782847156390483Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1206200.9347256628225538Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1156430.8961571864184097Illumina RNA PCR Primer (100% over 30bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1041750.8072877294357449Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG850970.659445777890987Illumina Small RNA Adapter 1 (100% over 26bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG809450.6272705088473853Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC785680.6088503223067685Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG776480.6017209274319818Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT733320.5682747662585269Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG607490.4707647926613109No Hit
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA598620.46389112608094607Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC580130.4495625922510762Illumina RNA PCR Primer (100% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG579390.44898914092419123Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG542610.42048706010955555No Hit
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA539670.418208753486526Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA520400.4032757709607503Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT507510.39328686879379393Illumina RNA PCR Primer (100% over 31bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT480450.37231714865121535Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG449070.3479997126543892Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCTACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAG441450.3420947138559247No Hit
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG409890.31763778969850487Illumina Small RNA Adapter 1 (100% over 26bp)
GGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT388780.3012789281977719Illumina RNA PCR Primer (100% over 31bp)
GCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC374400.2901353740347904Illumina RNA PCR Primer (100% over 30bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT365880.2835329344333576Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT361370.2800379810762612Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC355340.27536512769637395Illumina RNA PCR Primer (100% over 32bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA351660.27251336974645934Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA346810.268754938752686Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA345630.2678405163665721No Hit
TACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAGAAC344370.26686409924241655No Hit
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG327680.2539304470184832Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACGTGT324520.2514816548658391Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT312210.24194221454968456Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT308420.2390052138349627Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT297540.23057392946130212Illumina RNA PCR Primer (100% over 35bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA283980.22006582136324723Illumina RNA PCR Primer (100% over 45bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC280090.21705132722597337No Hit
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA277440.21499775152834466Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT268310.20792260204934454No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG266490.20651222175889766Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTG263460.20416417105557122Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA260420.2018083710099896Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC255560.1980421906739611Illumina RNA PCR Primer (100% over 30bp)
TGATCAAGTTTGAACAACTTCCCGGCGATCGTCGGACTGTAGAACTCTGA252170.19541516364944733Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTCTTGTCCCCCATGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG251970.1952601768043433Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT232560.18021870348699479Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC230240.17842085608378774No Hit
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG229750.1780411383132828Illumina Small RNA Adapter 1 (100% over 26bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT227850.17656876328479432Illumina RNA PCR Primer (100% over 44bp)
ACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTC226460.1754916047113211Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGCACGAGACAGAGTGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACT226240.17532111918170665No Hit
TGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAG220510.17088074606947548Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT217960.16890466379439878Illumina RNA PCR Primer (100% over 50bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA201230.15594001420144463Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAAC200940.15571528327604375Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCTCGCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAA199570.15465362338708097Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC197720.15321999506986847Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCACTGCAAGCAGTCCACCACGGTCGATCGTCGGACTGTAGAACTCTGA195950.15184836149069758Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG195670.15163137990755188Illumina Small RNA Adapter 1 (100% over 26bp)
TGCACGAGACAGAGTGTCGGCGCCAGTCAGGAGGATCGTCGGACTGTAGA193400.14987227921562088No Hit
CGGGCCATCCCGAAATCCAACTACGGATCGTCGGACTGTAGAACTCTGAA188590.14614484559086835Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA184730.1431535994803601Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT179790.13932542440628995Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAAC178250.13813202569898875Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT169060.13101038016645744No Hit
AAGCCTACAACACGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA163190.12646151626265342Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC162390.1258415688822372Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT160560.1244234392495351Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAAC156300.12112221944881874Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT154360.11961884705130939Illumina RNA PCR Primer (100% over 31bp)
CCTCGCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC151990.11778225293682634Illumina RNA PCR Primer (100% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA151700.11755752201142548No Hit
TCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGA149030.11548844762928635Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGTCGTACGAGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTC146730.1137060989105897No Hit
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT145790.11297766073860066Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGT143790.11142779228756011Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT141720.10982367844073315No Hit
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA137020.10618148758078785Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC135730.1051818224298667Illumina RNA PCR Primer (100% over 30bp)
TGGGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTG130970.10149313551639022Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)