FastQCFastQC Report
Wed 30 Apr 2025
1H9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12904321
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA7673295.946295043342459Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC7335975.68489423038996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT5562664.310695618932605Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3659742.8360577825055655Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3618452.8040607483338333Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2357991.8272871544345495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA2319451.7974211893829983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1935991.5002649112649942Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG1591351.233191579781687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1440731.1164709867338234Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTAACTGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1185220.9184675427711385Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1105870.8569765119761048Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1073120.8315974160903158Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC983960.7625042805429283Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC811800.629091604277358Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT768500.5955369523123301Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC753840.5841764165662029Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA734840.5694526662813176Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG695080.5386412814746316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA606960.47035407752178515No Hit
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA553920.4292515662001899Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC548960.42540789244160926Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCGACTGCTGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG543640.4212852423618414Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA534930.41453556525755986No Hit
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC513180.3976807458524939Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC490490.3800974882754389Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG479910.3718986841694344TruSeq Adapter, Index 11 (100% over 35bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG465870.36101860764312976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAGGTAGATCGGAAGAGCACA430030.3332449650004832No Hit
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC414950.3215589568796375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC408180.3163126521728652Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCGACTGCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG377050.2921889497324191TruSeq Adapter, Index 11 (100% over 35bp)
ATGGGGGACAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG352730.27334254936776603Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC348140.26978560127262796No Hit
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC348000.26967711048105514Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT335260.2598044484479269TruSeq Adapter, Index 9 (97% over 36bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC334370.2591147569872138Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC332220.2574486484023452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAAGATCGGAAGAGCACACGT330630.256216502983768No Hit
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC329530.2553640753356957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA313770.2431511119414962Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAG302750.23461133677626275Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC290000.2247309254008793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG287300.22263860299197455Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC280710.21753178644579596TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA272500.21116957645427448No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG262870.20370695986251428No Hit
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT259360.20098694073093812Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCCGAGGTCGCGGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA245970.19061057145122165Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC244890.18977364248765977Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT240270.18619344636575608Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGACCGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG239400.18551925358955346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGGGAAGTTGTTCAAACTTGATCAAGATCGGAAGAGCACACGTCTGAA237700.184201865406169Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCATGGGGGACAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC236620.1833649364426071Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG227640.17640602709743505No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCT218040.16896665853244042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGACTGGCGCCGACACTCTGTCTCGTGCAAGATCGGAAGAGCACACGTC217950.16889691445214358No Hit
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG216480.16775776114062876Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTAGGGGG215430.1669440802038325TruSeq Adapter, Index 22 (97% over 37bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC214670.1663551301924371Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC211560.16394508475106906No Hit
CGTGGTGGACTGCTTGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC204460.1584430517498751Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC199370.1544986365419769No Hit
CGTGGTGGACTGCTTGCAGTGCTCAGATCGGAAGAGCACACGTCTGAACT192790.1493995693380535Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT189270.14667180086422213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCTGACTGGCGCCGACACTCTGTCTCGTGCAAGATCGGAAGAGCACAC189010.14647031796558688No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA187960.1456566370287906Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCGTGGTCGAGGCGAGGAGATCGGAAGAGCACACGTCTGAAC185490.1437425494917555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC185370.14364955738469307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAA185160.1434868211973338Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTAGTTGGATTTCGGGATGGCCCGAGATCGGAAGAGCACACGTCTGAAC177660.13767481450593178Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA176330.1366441519859898Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTAGGGG172800.13390863416990326TruSeq Adapter, Index 22 (97% over 37bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG168820.13082439595233256Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACT168420.13051442226212445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTCAGATCGGAAGAGCACACGTCTGAACT161470.12512862939475855Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCGTGTTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA153160.11868892598068508Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC150280.11645711541118668No Hit
GAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC149340.11572867723919764Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGACCGAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACT147500.11430279826424032Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG145410.11268318573290297TruSeq Adapter, Index 11 (100% over 35bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTCGTACGACGAGATCGG145080.11242745743848127No Hit
GTGGTCGAGGCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG140140.1085992823644111Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA138650.1074446303683859No Hit
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC138630.10742913168387551Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA138160.10706491259788098Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGACCGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCC134260.10404266911835192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA131840.10216732829259284Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)