FastQCFastQC Report
Wed 30 Apr 2025
1H5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16315491
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG6195643.7973972098050868Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG4310312.641851232059152Illumina Small RNA Adapter 1 (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG3365452.0627328959943654Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT3134171.9209780447306184Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA2937601.8004974536163207Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG2835461.7378943728999636Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2417971.4820087241015303Illumina RNA PCR Primer (100% over 30bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA2347291.4386879316105166Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC2012891.23372934348099Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1764151.0812730061265088Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT1537320.9422456241126914Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG1506010.9230552730530757Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1498460.9184277690447686Illumina Small RNA Adapter 1 (100% over 26bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1082850.6636943993901255Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT968170.5934053716189112Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG920950.5644635518477501No Hit
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG767160.47020344039906614No Hit
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG753520.4618432874622038Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC647960.3971440393672492No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT634370.3888145321522962No Hit
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA630420.38639352012146005Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA597530.36623476424950985Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGA560350.34344660543774014Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT547910.33582194982670155Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG541400.33183187683410814Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG538080.32979700089932934Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA521010.3193345514394878Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT512840.31432704047950505No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA507790.3112318225666638No Hit
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG497500.3049249329977259Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG474860.29104855011718617Illumina RNA PCR Primer (100% over 33bp)
GAGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAAC473150.29000046642788746Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA451450.27670022311924297Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG444540.272464984351375Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA424640.260267987031466Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA413510.25344624933445153No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA382950.23471558410347568No Hit
GAGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTA371970.22798578357218915No Hit
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG370900.22732996512332973Illumina RNA PCR Primer (100% over 34bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC364200.22322343838748096No Hit
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT349110.21397455951524844Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC337400.20679733144408585Illumina RNA PCR Primer (100% over 30bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG328430.20129948893355404Illumina Small RNA Adapter 1 (100% over 26bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT306100.18761310952885207Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT301770.18495919001150501Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG294590.18055846434532677Illumina Small RNA Adapter 1 (100% over 26bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA291320.1785542341324573Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC290230.17788615739483413Illumina RNA PCR Primer (100% over 30bp)
GGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT288100.17658064964149717Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA283750.17391447183538639Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT283500.17376124322583977No Hit
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC278010.17039634296019654Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTC277960.1703656972382872Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA276000.16916438493944191Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT275220.16868631167765652Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG269580.1652294742462853Illumina RNA PCR Primer (100% over 37bp)
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA260880.15989711863406378Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT260480.15965195285878922Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT257730.15796643815377667Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA257560.157862242699285Illumina DpnII expression Sequencing Primer (100% over 24bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA228580.14009998228064358Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC228040.13976900848402296Illumina RNA PCR Primer (100% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT227200.1392541603559464Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG225410.13815704351159275No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG220930.13541118682851774Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT218320.13381148014485128Illumina RNA PCR Primer (100% over 31bp)
CTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTA214330.1313659515364876Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT212360.13015851009326046Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA206280.12643199030908722Illumina DpnII expression Sequencing Primer (100% over 25bp)
ATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC204670.12544519806360716Illumina RNA PCR Primer (100% over 30bp)
ACTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT202650.12420711089847067Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA195290.11969606063341888Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT190610.1168276210627066Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCTACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAG189110.11590824940542702No Hit
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT188220.11536275555544115Illumina RNA PCR Primer (100% over 35bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT182620.11193043470159739Illumina RNA PCR Primer (100% over 50bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT180960.11091299673420801Illumina RNA PCR Primer (100% over 44bp)
GACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC180780.11080267213533444Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAAC178120.10917231972975866No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG177240.10863295502415465Illumina DpnII expression Sequencing Primer (100% over 23bp)
TAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT173880.10657356251184841Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT170190.10431190823494065Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGA166240.10189089620410442Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG165320.10132701492097296Illumina Small RNA Adapter 1 (100% over 26bp)
GCCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG164830.10102668684626163Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA164310.1007079713384047Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA163450.10018086492156442Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)