FastQCFastQC Report
Wed 30 Apr 2025
1H5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16315491
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC74

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA5806923.5591451093932753Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC4182922.563772061778588Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA3248021.9907583535181381Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2963991.8166722656400593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2725541.6705228178545164Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA2696481.65271152428082Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2289531.403285993660871Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2205541.3518073099975967Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1956731.1993080686324427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1636571.0030773821026902Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1460080.8949041129071751Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1439930.8825538869777195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1437640.8811503129142728Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA1073950.6582394608902667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG944710.5790263988990586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA922660.5655116355370489No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA773450.4740586722152585No Hit
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC729430.4470781786462939Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG668780.4099049179702897No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA633370.3882016177141098No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA630840.3866509441854983No Hit
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA607820.3725416538184477Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT561580.3442004901967094Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC547220.3353990388643529Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCTGAA543230.33295351025598924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC522000.31994133673329234Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG520590.3190771273754495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC503910.3088537145465006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG498300.305415264548275No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA477370.292586965357034Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCTCAGATCGGAAGAGCACACGTCTGAACT460580.2822961319398846Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA449120.27527213247826865TruSeq Adapter, Index 1 (97% over 37bp)
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT426310.26129155414323724Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC416310.2551624097613734Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC414860.2542736838260032No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA405080.24827938062054034Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC385310.2361620621775955No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTCAGATCGGAAGAGCAC378420.23193908169849134No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT365200.22383635282566733No Hit
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAG348660.21369874801806457TruSeq Adapter, Index 1 (97% over 37bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC335310.20551634026827634Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC325170.1993013878650664Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGGGACAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC319480.1958139047117859Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGTGGACTGCTTGCAGTGCAAGATCGGAAGAGCACACGTCTGAACTC297360.18225623733910307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG292890.17951650980040992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC285770.17515255900052287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC283420.17371221007078488Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTAGATCGGAAGAG280620.171996049643863No Hit
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA278510.17070280017928974Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCT275650.1689498648860767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA275490.16885179857596685Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC275460.16883341114282127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC265660.1628268496485947Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT265330.1626245878839932Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC259880.1592842041958774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA254990.156287052593146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG252960.1550428362836276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAT252780.15493251168475408TruSeq Adapter, Index 1 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG251990.15444830927858683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA249940.15319183468030473Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCTAGATCGGAAGAGCACA226270.13868414992843303No Hit
AGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC225590.1382673681104663Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG222750.13652669110601698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT217640.13339469832688455Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA216340.13259790955724224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT206290.1264381194534691TruSeq Adapter, Index 12 (100% over 35bp)
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC203440.12469131330463791Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT201090.12325096437489991Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC198390.12159609539179667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAG194070.1189483050188315Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAATCGGCCGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC192100.11774086357560432Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAGGTAGATCGGAAGAGCACA188880.11576727908464415No Hit
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA187700.11504404004758423Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTCAGATCGGAAGAGCACACGTCTGAACT184090.11283141892573138Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC179540.11004265823198332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGA176230.10801391144158641TruSeq Adapter, Index 1 (97% over 37bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGAGATCGGAAGAGCACACGT174650.10704550662925191No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA173690.10645710876859299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC170660.10459997802088825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC164380.10075087534907777Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG164160.10061603417267677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC163800.10039538497492964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)