FastQCFastQC Report
Wed 30 Apr 2025
1H4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15304850
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA272164617.782898884993973Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC6709294.383767237182985No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC5101533.3332767064035256No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG4402482.8765260685338307Illumina RNA PCR Primer (100% over 36bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3810082.489459223710131Illumina RNA PCR Primer (100% over 37bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG3538382.311933798763137Illumina Small RNA Adapter 1 (100% over 26bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT2079531.358739223187421Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1406420.9189374610009244No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1060400.6928522657850289No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG965530.630865379275197No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT814430.5321385051143919No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA773380.5053169420151129Illumina RNA PCR Primer (100% over 45bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG739600.4832455071431605Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT719900.4703737704061131No Hit
GAGGTCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG709560.4636177420882923Illumina RNA PCR Primer (100% over 36bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG709310.4634543951753856Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG628640.4107456133186539Illumina Small RNA Adapter 1 (100% over 26bp)
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG624230.4078641737749798Illumina RNA PCR Primer (100% over 37bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC596990.3900658941446665Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA586280.38306811239574384Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG569470.3720846659718978Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT551480.3603302221191322Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC527840.3448841380346753Illumina RNA PCR Primer (100% over 32bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC440990.28813742049089014No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG433800.28343956327569364Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT424630.27744799851027613No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT408940.2671963462562521Illumina RNA PCR Primer (100% over 50bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC394500.25776142856676154No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA382460.2498946412411752Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA352930.2306001038886366No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT344390.225020173343744Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT339480.2218120399742565Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG331920.21687242932795814Illumina RNA PCR Primer (100% over 36bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA309740.2023802912148763Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG308440.20153088726776155Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC292010.19079572815153367No Hit
GGCCATTACCAGTTTAGAATCCATGATCAGATCGTCGGACTGTAGAACTC291640.19055397472043176Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG280930.18355619297150905Illumina RNA PCR Primer (100% over 37bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG280390.18320336363963058No Hit
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC269650.17618598026115903Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG259560.1695932988562449No Hit
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG253420.16558149867525654Illumina Small RNA Adapter 1 (100% over 26bp)
GATAACCTGAGATTATCAGTCCTCTTTAGATCGTCGGACTGTAGAACTCT253100.16537241462673596Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG251270.16417671522425897No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT240450.1571070608336573Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCTCAGAGCATGTTTCTTATCACTGGACATCAGCGTATATTAAAGACA233090.15229812771768425No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT223430.14598640300296967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205460.13424502690323656No Hit
GATCAAGTACGTGGGTATCCAATCAGACGAGATCGTCGGACTGTAGAACT204700.1337484522880002No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT177020.11566268209097115No Hit
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA173210.11317327513827315Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTCTGAATCACTGTCCATCCTGATCGTCGGACTGTAGAACTCTGAACGT169260.11059239391434741Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT163650.10692688918872123Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACACGTCTTATCATTAGCCCAAAGCAAGAGATCGTCGGACTGTAGAACTC159980.10452895650725097Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG158350.10346393463509934Illumina RNA PCR Primer (100% over 36bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC157800.10310457142670461Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGACAGTCGCCGCAGTGAAAACACGCACAGATCGTCGGACTGTAGAACT155660.10170632185222332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)