FastQCFastQC Report
Wed 30 Apr 2025
1H4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15304850
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC257046916.79512703489417Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6600234.312508779896569No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG5034163.28925798031343No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAA4101682.6799870629244977TruSeq Adapter, Index 7 (97% over 35bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAT3512662.2951286683632968TruSeq Adapter, Index 7 (97% over 35bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC3358112.1941476068043793Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG2002061.308121281815895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1366550.8928868953305651No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1044540.6824895376302282No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC843090.5508645952100151No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG801840.5239123545804107No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA704770.46048801523700006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC688970.450164490341297No Hit
CCAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAA669890.4376978539482582TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA656530.4289685949225246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTGGGGGG640080.4182203680532641TruSeq Adapter, Index 7 (97% over 35bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC606100.3960182556509865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAT591490.3864722620607193TruSeq Adapter, Index 7 (97% over 35bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC566430.3700983675109524Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT561050.3665831419452004Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA546950.3573703760572629Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC505960.3305880162170815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC491930.3214209874647579TruSeq Adapter, Index 7 (97% over 35bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG419010.2737759599081337No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA417150.2725606588761079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG411170.2686534007193798No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT390950.25544190240348646No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT352890.23057396838257155Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG344210.22490256356645114No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC326670.2134421441569176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG321080.20978970718432394Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAA306430.20021757808799173TruSeq Adapter, Index 7 (97% over 35bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC284130.1856470334567147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA282680.1846996213618559No Hit
TGATCATGGATTCTAAACTGGTAATGGCCAGATCGGAAGAGCACACGTCT280920.18354965909499277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA280350.18317722813356552Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA278220.18178551243560048No Hit
AGGCAATGATGTCTTTAATATACGCTGATGTCCAGTGATAAGAAACATGC267120.17453290950254333No Hit
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT263520.17218071395668694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAT256230.16741751797632776TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC251290.1641897829772915No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA248130.1621250779981509No Hit
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC248100.16210547636860212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAGAGGACTGATAATCTCAGGTTATCAGATCGGAAGAGCACACGTCTG240100.15687837515558795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC227080.14837126793140737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTGGGGGGGGGGG225960.14763947376158537TruSeq Adapter, Index 7 (96% over 31bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC218430.1427194647448358No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTTGAACT208670.1363424012649585No Hit
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTCCGAGG199660.1304553785238013No Hit
TCGTCTGATTGGATACCCACGTACTTGATCAGATCGGAAGAGCACACGTC197960.12934461951603576No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACAGTCTGAACT172600.11277470867078083No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACTCTGAACT170900.11166394966301532No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC170830.11161821252740145No Hit
AGGATGGACAGTGATTCAGAAAAGATCGGAAGAGCACACGTCTGAACTCC158470.10354234115329455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC154550.1009810615589176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)