FastQCFastQC Report
Wed 30 Apr 2025
1H10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10880563
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG4549264.181088791085535Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG4179423.841179909532255Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3720883.4197495111236433Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT2691372.473557664249543Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2652142.437502544675308Illumina RNA PCR Primer (100% over 30bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA2452012.2535690478516597Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1910001.7554238691508885Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1878821.7267672637895668Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1567421.4405688382117727Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1281031.177356355548881Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1176281.0810837637721504No Hit
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG864100.794168463525279Illumina RNA PCR Primer (100% over 36bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG812230.7464962980316368Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG795740.7313408322712713Illumina Small RNA Adapter 1 (100% over 26bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT784980.7214516381183583Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA701870.6450677230580807Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG669360.6151887544789731Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC569310.5232357921184777No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC561300.5158740407091067Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG451150.41463847045414837Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA442430.40662417928189926Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT420930.38686417237784476Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT414220.38069721208360263Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG386280.3550183938092174Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG373850.34359435260840826Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA371670.34159077981534597No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG362710.3333559118218423Illumina RNA PCR Primer (100% over 37bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA343130.31536051948782434Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG318200.29244810218000666Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG317830.29210804624723924Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG311740.28651090940790475No Hit
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG310630.28549074160960236Illumina Small RNA Adapter 1 (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA309410.2843694761015583Illumina RNA PCR Primer (100% over 45bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA304050.2794432604268731Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC292510.26883719160488295Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT278330.25580477774909255Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC273060.2509612783823778Illumina RNA PCR Primer (100% over 30bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG256350.23560361720252895Illumina RNA PCR Primer (100% over 33bp)
TAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA246660.226697828044376Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT244270.22450125053271602Illumina RNA PCR Primer (100% over 50bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG235360.21631233604364036No Hit
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA227830.2093917382767785Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT225860.20758117020231395Illumina RNA PCR Primer (100% over 31bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT224540.2063679976854139Illumina RNA PCR Primer (100% over 35bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT220980.20309610817013787Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT209460.19250842074991892Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC208480.19160773206312945No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG208370.19150663435338777Illumina RNA PCR Primer (100% over 37bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT207380.1905967549657127Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG203380.18692047461147002Illumina RNA PCR Primer (100% over 34bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA199580.18342800827493946Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC198300.1822515985615818Illumina RNA PCR Primer (100% over 32bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT195690.17985282563043842Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA192500.17692099204792988Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC182390.16762919345258145Illumina RNA PCR Primer (100% over 30bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG179360.1648444110842426Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA176590.16229858693892954Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT176420.16214234502387423Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA171650.1577583807014398Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA170740.15692202692084958Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT165160.15179361582668105No Hit
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT161200.14815409827598075Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT160560.14756589341930193No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT158020.14523145539435783Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG156410.1437517525517751Illumina Small RNA Adapter 1 (100% over 26bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA155280.14271320335170157No Hit
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG154440.1419411844773106Illumina Small RNA Adapter 1 (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT150960.13874282056911946No Hit
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT150940.13872443916734825Illumina RNA PCR Primer (100% over 31bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT142110.13060905028535746Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT137700.1265559511948049Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC135210.12426746667428881Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA133840.1230083406529607No Hit
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA133700.1228796708405622Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGT131580.12093124225281357Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT130240.11969968833414227Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG128200.11782478535347848Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC126710.11645537092152308Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCAGTCCGGGCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC126670.11641860811798066Illumina RNA PCR Primer (100% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC122310.1124114625318561Illumina RNA PCR Primer (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119540.10986563838654305No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT118580.10898333110152479No Hit
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG117190.10770582367842546Illumina Small RNA Adapter 1 (100% over 26bp)
CAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTCG117000.10753120036159894No Hit
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT112780.10365272458787288Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG111750.10270608239665538Illumina Small RNA Adapter 1 (100% over 26bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT109600.10073008170624995Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC109510.1006473653982795Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)