FastQCFastQC Report
Wed 30 Apr 2025
1H10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10880563
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAT4195593.8560412728642808TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA3807163.499046878364658Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3489953.207508655572327Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2471942.2718861147166742Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2443742.245968338219263Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2197882.0200057662457356Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1781501.6373233627708417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1758691.616359374050773Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1470291.3512995605098743Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1150381.057279848478429Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1149441.056415922595182No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAA803850.7387944906894983TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA787420.7236941691344465Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC748540.6879607240912075Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG729550.6705075831094403Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC657440.6042334390233299Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC621560.5712572042457729Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG561150.5157361801958226No Hit
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT524860.48238312668195565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA420640.3865976420521622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA408690.3756147544938621Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG398410.3661667139834584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG394060.3621687590982195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC365650.3360579778822107No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA353090.3245144575698886Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC348590.3203786421713656Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAT333790.3067764048606676TruSeq Adapter, Index 7 (97% over 35bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA315120.2896173663072398Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA304720.2800590373862088No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA301920.2774856411382389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC298120.2739931748017083Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC297580.2734968769538856Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT283170.26025307697772626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT280260.2575785830200147Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC267750.24608101621212067Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC264490.24308484772341282Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTGGGGGG246320.22638534421426537TruSeq Adapter, Index 7 (97% over 35bp)
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACT236310.217185452627773TruSeq Adapter, Index 7 (97% over 35bp)
GAGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA236060.21695568510563287Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA213770.19646961283161543Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCA207710.19090004809493774TruSeq Adapter, Index 7 (97% over 35bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA207630.1908265224878529TruSeq Adapter, Index 7 (100% over 35bp)
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC207260.19048646655508542Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT204380.1878395447000307No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC197430.181452007584534No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG196470.18056970029951575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG194230.17851098330113985Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAT190780.1753401914956055TruSeq Adapter, Index 7 (97% over 35bp)
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTC186720.17160876693604918TruSeq Adapter, Index 7 (97% over 35bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC184700.1697522453571566TruSeq Adapter, Index 7 (97% over 35bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC183210.1683828309252012Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT182350.16759243064903903Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA181510.16682041177464804Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG180130.16555209505243432No Hit
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC166260.1528045929240978Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT164830.151490322697456Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC163860.15059882471155217Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG162510.14935808009199522No Hit
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC161520.1484482007043202Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA160050.14709716767413597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC159180.1462975766970882Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA157660.14490059016247597No Hit
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC152320.13999275588956198No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG151180.1389450159886028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC149450.13735502473539282Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC147030.135130875121076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC146110.1342853306396002No Hit
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC144260.13258505097576292Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA139130.12787022142144666TruSeq Adapter, Index 7 (100% over 35bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC127730.11739282241185497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG125770.11559144503827605Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGCTCTTAACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC124900.11479185406122827Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT124590.11450694233377445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT122820.11288018827702206Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGACCGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCC122120.11223683921502958Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT121280.11146482034063863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA121200.11139129473355376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC119190.10954396385554682Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG116410.10698894900934813No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGAGATCGGA115210.10588606490307532No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTGGGGGGGGGGG114110.10487508780565857TruSeq Adapter, Index 7 (96% over 31bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC112480.10337700356130468TruSeq Adapter, Index 7 (97% over 35bp)
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC111230.10222816595060384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC111130.10213625894174777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)