FastQCFastQC Report
Wed 30 Apr 2025
1G9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868698
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT282924417.829087175267937Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA12320717.764159353212217Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG8591045.4138279019488555Illumina RNA PCR Primer (100% over 37bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG5286853.3316217877484338Illumina RNA PCR Primer (100% over 36bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG2260361.4244142777183106No Hit
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT1570270.9895392804122934Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1368570.8624337043908705No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1288470.8119569734076482No Hit
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA1171630.7383277443429827Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1075680.6778627963050277Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG916290.5774197731912221No Hit
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG899630.5669211172838503No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG867190.5464783563213567Illumina RNA PCR Primer (100% over 37bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT849130.5350974604217686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG796290.5017992024298401No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA645080.40651098155626886Illumina RNA PCR Primer (100% over 45bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG601220.37887166294298374Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG546610.3444580015323248Illumina RNA PCR Primer (100% over 36bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG538860.33957417300398557Illumina DpnII expression Sequencing Primer (100% over 23bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG533500.33619645417664384Illumina RNA PCR Primer (100% over 37bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC523630.3299766622315202Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCGATCGTCGGA522230.3290944222393041No Hit
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT504130.31768831948279563Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA483550.3047193915972186Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCGATCGTC469590.2959221985319779No Hit
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG459650.28965829458724335Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT429170.2704506696138524Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT399230.2515833372088876Illumina RNA PCR Primer (98% over 50bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA398400.25106029492778803No Hit
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC385940.24320835899706456Illumina RNA PCR Primer (100% over 32bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT382430.24099645730229413Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT361010.22749818542138742No Hit
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG336060.2117754084172501Illumina RNA PCR Primer (100% over 36bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA319310.2012200370818072Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTA308790.19459063371172605Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC308190.19421253085791915No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG304110.19164143145203216No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC302240.19046301089100062No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT301820.19019833889333582No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC298520.1881187731973978Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTGATCGTCGGACT281060.17711598015161673No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTGATCGTCG271540.1711167482045471No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG261610.16485914597404275Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTA247780.15614387519379347No Hit
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT240620.151631847805031Illumina RNA PCR Primer (100% over 35bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT233870.1473781906997033No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG211950.13356483310729084Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG208760.1315545862678841Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCGTCAAAAGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC199920.1259838708884623Illumina RNA PCR Primer (100% over 32bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT194460.12254313491881944Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG189760.1195813292306653Illumina Small RNA Adapter 1 (100% over 26bp)
AGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC183390.11556713726608193Illumina RNA PCR Primer (100% over 32bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC182120.11476681955885733No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG164900.103915267654599Illumina Small RNA Adapter 1 (100% over 26bp)
AACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG160900.10139458196255294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)