FastQCFastQC Report
Wed 30 Apr 2025
1G9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868698
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGGGGG193295512.180930029672252TruSeq Adapter, Index 7 (96% over 32bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC11739137.39766425701718Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT8219835.17990196801275TruSeq Adapter, Index 7 (97% over 36bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA5096513.211674959092422TruSeq Adapter, Index 7 (97% over 36bp)
AGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAA2217351.3973106048145854No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGGGGT2096681.3212678191997858TruSeq Adapter, Index 7 (96% over 32bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG1498110.9440661105277824Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1351340.8515758507723822No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1266650.798206632957537No Hit
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC1124440.7085899548910691Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1040740.6558446067850052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC881670.5556032385265635No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGGGTG859570.5416764500780089TruSeq Adapter, Index 7 (96% over 32bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG833560.5252856913654793No Hit
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT824290.5194440022741627TruSeq Adapter, Index 7 (97% over 36bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC757860.47758171464350757No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGG601110.37880234408645247TruSeq Adapter, Index 7 (97% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA576250.36313628251038615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA524900.33077697993874483TruSeq Adapter, Index 7 (97% over 36bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA515520.3248659719908968Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAG506580.31923223946917384No Hit
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT504070.31765050919741494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT496370.31279818924022623TruSeq Adapter, Index 7 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGTGGG488250.30768119728537274TruSeq Adapter, Index 7 (96% over 32bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC482610.3041270304595878Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT460730.29033887972409583Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGG459520.2895763723022519No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA440130.2773573484100586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG411400.2592525234269377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG385840.24314534185476336No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG367200.2313989465298287TruSeq Adapter, Index 7 (97% over 36bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC358370.22583453286463703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG356030.22435993173479007No Hit
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA314560.1982267228225025TruSeq Adapter, Index 7 (97% over 36bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC310100.19541615827587114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT301950.1902802611783273No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA297870.18770916177244032No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC295350.1861211297864513No Hit
GTTGGATCCGGCCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGT294450.18555397550574093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA292120.1840856760901241No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT283030.1783574178549494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAG272550.17175322134178872No Hit
AAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGA267090.16831248537214583No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA252090.15885991402697308Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCAC247090.1557090569119155No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGGGTT245290.15457474835049478TruSeq Adapter, Index 7 (96% over 32bp)
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG230710.14538684900298687TruSeq Adapter, Index 7 (97% over 36bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC224030.14117730389726996No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGAGGG212050.13362785024959198TruSeq Adapter, Index 7 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGTGGGG209980.13232339540395816TruSeq Adapter, Index 7 (96% over 32bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA199350.12562467317734574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA198510.12509532918201607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTTTTGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG190450.12001614751254325TruSeq Adapter, Index 7 (97% over 36bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG186700.11765300467625006Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATTTGGGGGGGGGGG178810.1126809521486892TruSeq Adapter, Index 7 (96% over 32bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC176400.11116223901923145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT175720.11073372245158361No Hit
TCCGGCCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG174730.1101098527428022TruSeq Adapter, Index 7 (97% over 36bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC159660.10061316939801866Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)