FastQCFastQC Report
Wed 30 Apr 2025
1G8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485434
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT9157407.973055262865991Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA5879345.1189532759493455Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT3221282.804665457134663Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2609602.272095246901423Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG2438432.1230630031046283Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTGATCGTCGG1997861.7394727965873995No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1909211.6622880772289492Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1335411.1626987713307133Illumina RNA PCR Primer (100% over 45bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1262471.0991922464575568Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1160701.0105843627676585Illumina RNA PCR Primer (100% over 37bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG1044350.9092821394472338No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA999540.870267505781671Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG975830.8496239671918362Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT817840.7120671278072731No Hit
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT795780.692860191439Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT708470.6168421672180607No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC674560.5873178148949356No Hit
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTA647590.5638358985825002No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCGATCGTC576320.5017833892911665No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT558770.4865031656618287Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT534380.465267572823108Illumina RNA PCR Primer (100% over 50bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG507540.4418988433523714Illumina RNA PCR Primer (100% over 36bp)
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG490250.4268449934064312Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCGATCGTCGGA428020.3726633229532293No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC421770.36722164787155626No Hit
CGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCT365680.31838587901858995Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCGATC360240.3136494450275018No Hit
CAGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCGATCG327350.28501317407770577No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCGATCGTCGGACTG307230.267495333654784No Hit
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG286020.24902846509761842Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGT280300.24404824406287126No Hit
CCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTG272220.23701324651728442Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAAC269820.23492364328592197Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTGATCGTCGGACT262520.2285677667905279No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG262080.22818467286477812Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTGATCGTCG232210.20217781931444645No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC225210.19608314322297268No Hit
CAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGA216450.18845609142849978Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG215120.18729810297111976No Hit
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG202600.17639733944751237Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG198290.1726447603111907Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA188740.16432988078639432Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA187890.1635898129752868Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCAGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGAT184950.1610300490168678No Hit
AGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTA184880.16096910225595307Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT184770.16087332877451563Illumina RNA PCR Primer (100% over 35bp)
TCAGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCG182270.1586966587418464No Hit
TCCAACCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAA181290.15784340408904007Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT179470.1562587883052569No Hit
CAGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGT177290.15436073203676937No Hit
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT172240.14996385857077757Illumina RNA PCR Primer (100% over 31bp)
AGTAACGTACCTCGCGGCAGACCGGCGATCGTCGGACTGTAGAACTCTGA171960.1497200715271186Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT170850.1487536300326135No Hit
AACCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGT169900.14792649542019917Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGATCGTCGGACTGTAGAACTCTGA166450.1449226907751157Illumina DpnII expression Sequencing Primer (100% over 24bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTC162800.14174475252741864Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACG158930.1383752673168467Illumina Small RNA Adapter 1 (100% over 26bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC146750.12777053091768237Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAGCACTGCAAGCAGTCCACCACGGTCGACGACCGGCCGGAAGGGATCG144780.12605531493193903No Hit
ACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC136050.11845438317785814Illumina RNA PCR Primer (100% over 30bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT135950.11836731637655139Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT133610.116329953225973Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCCGAGGGTCACAGCTGATCGTCGGACTGTAGAACTCTGAACGTGTAG130990.11404880303173567Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT128950.11227264028507761Illumina DpnII expression Sequencing Primer (100% over 22bp)
CACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC128820.11215945344337881Illumina RNA PCR Primer (100% over 32bp)
CGGATCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAA126210.10988700992927214No Hit
CCCTCGCCTCGACCACGGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGA125600.10935590244130088No Hit
TGCACGAGACAGAGTGTCGGCGCCAGTCAGGGATCGTCGGACTGTAGAAC124650.10852876782888657No Hit
TCTCTGCACGAGACAGAGTGTCGGCGCCAGTCAGGGATCGTCGGACTGTA123680.10768421985621092No Hit
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC120890.10525505609975208Illumina RNA PCR Primer (100% over 32bp)
TCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTC119290.1038619872788438Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCAGTTAAGAGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCCGA117760.10252986521885024No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA117010.10187686420904948No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC116440.10138058344160089Illumina RNA PCR Primer (100% over 32bp)
AACCGCTTCAAGTCTCAGCCCGAGATCGTCGGACTGTAGAACTCTGAACG116010.1010061961959818Illumina Small RNA Adapter 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)