FastQCFastQC Report
Wed 30 Apr 2025
1G8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485434
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG8719997.5922163672700576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC5650104.919361340633711Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC3045672.651767447359847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC2483862.162617450938293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA2321202.0209945919327033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAA1944971.6934231653762497No Hit
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1835321.5979544177433782Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1193461.039107446875756Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGG1118280.9736506256533275TruSeq Adapter, Index 15 (97% over 43bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAAT1118200.9735809722122821TruSeq Adapter, Index 15 (97% over 41bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC1001110.8716344545621871No Hit
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT947600.8250450091829356Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGA930560.8102088262402622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG798950.6956202090404245No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG759060.6608892619991548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA685480.5968255095976347No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG663530.577714346710799No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCAC641370.5584203435412193No Hit
GAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGG561420.4888104358964581No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG539680.4698821132923667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAA491440.42788108834198163TruSeq Adapter, Index 15 (97% over 39bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA476170.41458598778243816Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT415780.36200634647328084No Hit
GGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAG412570.35921150215133363No Hit
GGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTGAAGAT357670.31141182823391783No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGATCGGAAGAGCACACGTCTG346330.3015384529657303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTGAGATC324910.28288874412582055No Hit
GGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGC300000.26120040392030464No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCA282400.2458766468903134No Hit
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA275420.23979938415910101Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACT259170.22565102894675118Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAGATCGGAAGAGCACACGTCTGA258610.22516345485943326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAG252570.21990462006050449No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC252130.21952152613475467Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGA226630.1973194918015288No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGGG222610.19381940638899672TruSeq Adapter, Index 15 (97% over 39bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA216200.18823842442523286No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA213180.18560900702576846No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAA205310.1787568497629258Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC194340.1692056216595733Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCGGGCTGAGACTTGAAGCGGTCAGATCGGAAGAGCACACGTCTGAAC190200.16560105608547313Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA188610.1642166939446955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTGAGTAGA182150.1585921785802783No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTGAAGATC179720.1564764553085238No Hit
CCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA179240.15605853466225134TruSeq Adapter, Index 15 (100% over 39bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA177530.1545696923599056Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG176400.15358583750513913No Hit
GTTGGATCCGGCCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGT175740.15301119661651444Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTGAGATCG174180.15165295451612887No Hit
CGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAAC171920.1496852448065959Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT168220.14646377315824546TruSeq Adapter, Index 1 (100% over 35bp)
AAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTAGATCGGAAGAG166920.14533190474125748No Hit
TCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAA164910.14358186203499146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGGTCTGCCGCGAGGTACGTTACTAGATCGGAAGAGCACACGTCTGAA164740.14343384847276994Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACTCC161690.1407783110329135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCT157760.1373565857415575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCCGGCCGGTCGTCGACCGTGGTGGACTGCTTGCAGTGCTCCAGATC151450.13186267057910045No Hit
TCTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTC150330.13088752240446463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT144580.12588118132932546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCC131740.11470180404153643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAACGGACCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA129820.11303012145644649Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC129730.11295176133527039Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC127170.11072285122181713Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG124830.10868548807123875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGATCCGAGATCGGAAGAGCACACG121200.10552496318380307No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCAAGATCGGAAGAGCACACGT120540.1049503222951784No Hit
CGGACTGGCCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG120010.10448886824825253TruSeq Adapter, Index 15 (100% over 36bp)
CCTGACTGGCGCCGACACTCTGTCTCGTGCAGAGAAGATCGGAAGAGCAC119760.10427120124498561No Hit
TCCGGCCCGGACTGGCCGTGGTCGAGGCGAGGGAGATCGGAAGAGCACAC119700.10421896116420154No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC117250.10208582453218572No Hit
GGCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTCTTAACTGAGTAG117050.10191169092957218No Hit
TCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACTC116690.10159825044486782Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)