FastQCFastQC Report
Wed 30 Apr 2025
1G7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11782742
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA184653315.671504985851342Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG6713885.698062471367021Illumina RNA PCR Primer (100% over 36bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG5117674.343360823821824Illumina RNA PCR Primer (100% over 37bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4200573.565019076204843No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1942041.6482071830139369No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1237061.049891442925594Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1106300.9389155766968335Illumina RNA PCR Primer (100% over 45bp)
GAGGTCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1069680.907836223520807Illumina RNA PCR Primer (100% over 36bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT987890.8384211417002935No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC925660.7856066100742933No Hit
GAGGTCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG826260.7012459408854068Illumina RNA PCR Primer (100% over 37bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT811030.6883202568638098Illumina RNA PCR Primer (100% over 50bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG657540.5580534649744516Illumina RNA PCR Primer (100% over 36bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG504710.4283468143493255No Hit
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG492250.41777202623973264Illumina RNA PCR Primer (100% over 37bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT438860.3724599927589011No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG434510.36876815260828083Illumina Small RNA Adapter 1 (100% over 26bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC419720.3562158960961718Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA393610.3340563682035981Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC364120.3090282380790481Illumina RNA PCR Primer (100% over 32bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA352640.2992851748769514No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG348460.2957376135368151Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT343290.2913498403003308Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC337660.28657166557665437No Hit
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA334290.2837115503335302Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT327390.2778555280256497Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT303810.2578432083126322No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG301150.25558566927799997Illumina DpnII expression Sequencing Primer (100% over 23bp)
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG290330.24640274734013526Illumina RNA PCR Primer (100% over 36bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG269200.22846973989585784Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT265130.22501553543309358Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT259500.22023736070941724Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT252540.21433041646842477Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG236830.20099735698193172Illumina Small RNA Adapter 1 (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT219020.18588202983651853No Hit
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG213520.18121418596791816Illumina RNA PCR Primer (100% over 37bp)
GGCCATTACCAGTTTAGAATCCATGATCAGATCGTCGGACTGTAGAACTC205430.17434821198664963Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC205420.17433972499779762No Hit
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT204550.17360135696767356Illumina RNA PCR Primer (100% over 35bp)
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC185320.15728087740527627Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC183970.1561351339102562No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT176860.15010088483648373No Hit
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG166440.14125744245269903No Hit
GATAACCTGAGATTATCAGTCCTCTTTAGATCGTCGGACTGTAGAACTCT163930.13912720825084687Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGACAGTCGCCGCAGTGAAAACACGCACAGATCGTCGGACTGTAGAACT142580.12100748705182544No Hit
TGCGTGAGCCTAGAGATCCACCGTCAAAAGTTGATCGTCGGACTGTAGAA140540.11927614132601731No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC139660.11852928630704125No Hit
GATCAAGTACGTGGGTATCCAATCAGACGAGATCGTCGGACTGTAGAACT138830.1178248662323252No Hit
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG134560.11420092199252091No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG126770.1075895576768124No Hit
GCAGAAATACTCACCACTATACTACAGCGGAGATCGTCGGACTGTAGAAC123710.1049925390881002No Hit
ACCGTCAAAAGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC122160.10367705581604009Illumina RNA PCR Primer (100% over 32bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA121690.10327816733999608Illumina DpnII expression Sequencing Primer (100% over 25bp)
TATTGTAAATGGGCAAATTCAAGAAGATCAGATCGTCGGACTGTAGAACT119790.10166563945811595No Hit
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA118090.10022285135327584Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)