FastQCFastQC Report
Wed 30 Apr 2025
1G7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11782742
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC181933115.440641915099217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA6694715.681792913737736TruSeq Adapter, Index 7 (100% over 40bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT5068854.301927344246356TruSeq Adapter, Index 7 (100% over 41bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4250373.607284280687806No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1925541.6342036514081357No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1233291.0466918481283898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA1081600.9179527142323918TruSeq Adapter, Index 7 (100% over 40bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC994430.843971632409502No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT927270.7869730152794655No Hit
CAGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT838940.7120074427497436TruSeq Adapter, Index 7 (100% over 41bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA646410.5486074463821748TruSeq Adapter, Index 7 (100% over 40bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT479970.40735000392947585TruSeq Adapter, Index 7 (100% over 41bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC435620.3697102083708529No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGGGGG434850.36905671022924885TruSeq Adapter, Index 7 (97% over 45bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC433590.36798734963389673No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC430290.36518664331273654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT413510.35094547601907944Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC392450.33307187749676603Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG359190.3048441525750118TruSeq Adapter, Index 7 (100% over 36bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG352810.2994294536874354No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA345040.2928350633494309Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT334530.2839152380659782No Hit
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC333400.2829562083257021Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC328080.2784411302564378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT327510.2779573718918737Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA299880.2545078216937959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG299550.2542277510616799No Hit
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA270100.22923356889253793TruSeq Adapter, Index 7 (100% over 40bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA266810.22644134956022968Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC262030.22238456888897337Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG258320.2192358960248811Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG249260.21154668412496852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC229590.19485277705308324Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC218440.1853897844831025No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGGG218370.1853303755611385TruSeq Adapter, Index 7 (97% over 46bp)
TGATCATGGATTCTAAACTGGTAATGGCCAGATCGGAAGAGCACACGTCT206140.17495078819514168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG204870.1738729406109376No Hit
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC203630.1728205539932895TruSeq Adapter, Index 7 (100% over 39bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT196910.16711729748474508TruSeq Adapter, Index 7 (100% over 41bp)
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT186950.1586642565881524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA179850.15263849450323194No Hit
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG173430.1471898476602475No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA164800.13986557628097093No Hit
TAAAGAGGACTGATAATCTCAGGTTATCAGATCGGAAGAGCACACGTCTG162780.1381512045328668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGCGTGTTTTCACTGCGGCGACTGTCGCAGATCGGAAGAGCACACGTC142770.12116873984001346No Hit
TCGTCTGATTGGATACCCACGTACTTGATCAGATCGGAAGAGCACACGTC140250.11903001864930929No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCAAGATCGGAAGAGCACACG139890.11872448705063728No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT139190.11813039783099724No Hit
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC132280.11226588853426478No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA125370.1064013792375323No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTG123570.10487372124417219TruSeq Adapter, Index 7 (97% over 46bp)
AACTTTTGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG120310.10210696287841997TruSeq Adapter, Index 7 (100% over 36bp)
TGATCTTCTTGAATTTGCCCATTTACAATAAGATCGGAAGAGCACACGTC118240.10035015618605585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)