FastQCFastQC Report
Wed 30 Apr 2025
1G6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21447519
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG15144027.060965886077546Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA11509925.366550788461826Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA7401533.4509958937441665Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT7313923.410147346180227Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG4745952.212820046924775Illumina RNA PCR Primer (100% over 36bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA4727942.2044228052671264Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG4581152.1359813225949353Illumina RNA PCR Primer (100% over 37bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG4266581.9893116774951918Illumina Small RNA Adapter 1 (100% over 26bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC3954931.8440034952294484Illumina RNA PCR Primer (100% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT3055701.424733555428952Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT2517841.1739539664238086Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA2455411.1448457045311393Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC2380441.1098906125225954Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2264511.0558377404864405No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1934780.9020996787553843Illumina RNA PCR Primer (100% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1827650.8521498453970364Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT1731100.8071329835399609Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1724640.8041209801469344Illumina RNA PCR Primer (100% over 45bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1469490.6851561712102925Illumina RNA PCR Primer (100% over 31bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1358180.6332573944799863Illumina RNA PCR Primer (100% over 50bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA1325170.6178663368942581Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC1267200.5908375696042045Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT1128850.526331274027546Illumina RNA PCR Primer (100% over 35bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT1120190.5222935109650678Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1086390.5065341124071273No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1005820.46896799578543324Illumina RNA PCR Primer (100% over 32bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA1003170.4677324216381391Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG919630.4287815294626852Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT841630.39241368663666876Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG759060.353915061224564Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG726990.33896228277032886Illumina RNA PCR Primer (100% over 34bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC660840.3081195545274957Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA627970.29279377255709627Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG586830.2736120667383486Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG581820.2712761322183699Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG559590.2609112970129552Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT555470.2589903289047092Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA538760.251199217960828Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT527270.24584195495991867Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT519520.24222848339707728Illumina RNA PCR Primer (100% over 31bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT507610.23667539355018172Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC480760.22415646303891842Illumina RNA PCR Primer (100% over 32bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA477590.22267843660611747Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG472030.2200860621687758Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA464570.21660780438054397No Hit
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT421740.19663812863389935Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA407120.1898214893759973Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGA404460.1885812526847511Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC404350.1885299647012785Illumina RNA PCR Primer (100% over 30bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA398870.18597489061555325Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT398740.18591427754417655Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT387590.18071554103763704Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC375990.17530699005325512Illumina RNA PCR Primer (100% over 32bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT366920.1710780626887427Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG323810.15097783571144055Illumina RNA PCR Primer (100% over 33bp)
TTTCTGAATCACTGTCCATCCTGATCGTCGGACTGTAGAACTCTGAACGT320270.14932729515241366Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT313630.14623136596825023No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA305280.14233814176828563No Hit
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT295480.13776884869527334Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG291850.13607634524067796No Hit
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG289460.1349619972361372Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC287480.13403881353363062Illumina RNA PCR Primer (100% over 30bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT281930.131451101640241Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT271940.12679322023213965Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTG269810.1258000983703523Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC265990.1240190065806679No Hit
CCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA254820.11881094498622428Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC254770.11878763226646401Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTCGCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC254350.11859180542047779Illumina RNA PCR Primer (100% over 30bp)
GGCCAGTCCGGGCCGGATCCAGATCGTCGGACTGTAGAACTCTGAACGTG248420.11582691685691011Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG248310.11577562887343752Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC246380.11487575789069122Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG240640.11219945766221258Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT229990.10723384835327573No Hit
ACAAAATGGACAGGCTTCTTGTAGACAAGATCGTCGGACTGTAGAACTCT229930.1072058730895634Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTCTGA229750.10712194729842646Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT229500.10700538369962512No Hit
GCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGTA221650.10334528669726321Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGAA221440.10324737327427008Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTA215250.10036125856794904Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)