FastQCFastQC Report
Wed 30 Apr 2025
1G6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21447519
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA14258396.648037006051842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC10953055.10690770340383Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6873983.2050233875535907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6844943.1914833599168273Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC4492962.0948623474817762Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGA4485822.0915332911000104TruSeq Adapter, Index 10 (97% over 36bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAT4298492.0041898552461945TruSeq Adapter, Index 10 (97% over 36bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC4104091.9135500008182764Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3708671.7291836878661815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2839731.3240365936964549Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2393871.116152409050203Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2301141.072916638982812Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT2279321.0627429680794314Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2248271.0482657691083057No Hit
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1830700.8535719213024127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1738310.8104946777293915Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1622800.7566376325392229Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC1399770.6526489147765763TruSeq Adapter, Index 5 (97% over 35bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1246500.581186103623454Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT1204370.5615428059534532Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1067270.4976193283708013No Hit
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAG1066810.4974048513490068TruSeq Adapter, Index 10 (97% over 36bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC1055810.49227605300174815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA986640.4600252364853949Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG952520.44411663652098876TruSeq Adapter, Index 10 (97% over 36bp)
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC861500.4016781614693989Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG807130.3763279100020846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC726950.3389436325945206Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAA681430.3177197325247736TruSeq Adapter, Index 10 (97% over 36bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT649050.3026224152080248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT585240.2728707222499721Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA564650.2632705442526942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA555240.258883090393812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG540630.252071113679862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC523780.24421472712065204Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC518580.24179020426558429Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTGGGGGG510030.23780372918657863TruSeq Adapter, Index 10 (97% over 36bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC504770.2353512310677986Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTGGGGTG503510.23476375052983983TruSeq Adapter, Index 10 (97% over 36bp)
AGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC491150.22900084620510186TruSeq Adapter, Index 5 (97% over 35bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC480470.22402124926430883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC466060.21730252342939993No Hit
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG454170.21175875867040844TruSeq Adapter, Index 10 (97% over 36bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA450420.2100103046883884Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC443960.20699830129536195Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC395140.18423576172143732Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAAC391100.18235209396480778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG388580.1811771328888903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA385590.17978303224722636Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT380780.17754034860628867Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA379650.17701348113970666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC370070.1725467640336395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG352310.1642660859747927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG350770.16354805420617646TruSeq Adapter, Index 10 (97% over 36bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC307510.14337788906959356No Hit
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGA305620.14249666826265547TruSeq Adapter, Index 10 (97% over 36bp)
AGGATGGACAGTGATTCAGAAAAGATCGGAAGAGCACACGTCTGAACTCC302280.14093937858266964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC302270.14093471603871757No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA292100.13619290883947927No Hit
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC281540.13126926242611092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC277820.12953479607594706Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG265210.12365532815240775Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT264870.12349680165803793Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT259270.12088577704488802No Hit
CTTAACTGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC257580.12009780711699101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCCGAGGTCGCGGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA254290.11856383015676544Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT243770.1136588339192053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA242230.11294080215058908Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCTGTGACCCTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA238510.1112063358004252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGTCGAGGCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT238200.11106179693791156Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCA236010.11004069981241187Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT232990.10863261153889175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA230890.10765347730896055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG225110.10495852690467367No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC223240.1040866311856397No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGTAGATCGGAAGAGCACACGTCTG219240.10222161360481834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTGTAGAATAAGTGGGAGCAGCTAGATCGGAAGAGCACACGTCTGAA218270.10176934684146917Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGCTGTTCTTCCTCGCCGAGACAGATCGGAAGAGCACACGTCTGAAC217140.10124247937488713Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)