FastQCFastQC Report
Wed 30 Apr 2025
1G4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14191758
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA11765678.290495088769129Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG4173332.9406716208097685Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG3545332.498161256695612Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2290061.6136549115338634No Hit
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1953771.3766934300880835Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1749471.2327366348834303Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1621951.142881664132097Illumina RNA PCR Primer (100% over 36bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1583681.1159153080259683No Hit
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1331940.9385306598379144Illumina RNA PCR Primer (100% over 37bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1193780.8411783797327997Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC1008800.7108351199336967Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC963620.6789997405536369Illumina RNA PCR Primer (100% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT962990.6785558209208471Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC952940.6714742458263452Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG948600.6684161328004606Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT908070.6398573030909913Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA745480.5252908061143658Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG723190.5095845067256642Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG721770.5085839259660431Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG622100.43835302152136474No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG598510.42173069749357345Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA597940.42132905592104936No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT531500.37451315052018225Illumina RNA PCR Primer (100% over 50bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA481420.33922506288509147Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT461230.3249984956056889No Hit
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG458650.32318053901426447Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT427180.3010056964049133No Hit
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG403380.2842353991661921Illumina Small RNA Adapter 1 (100% over 26bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG391900.27614619696869125Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT382040.2691985023983639Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG361030.254394134961997Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGT359160.25307646875038314Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT358430.25256208568381733Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT321060.22622990048167396No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA316450.2229815361845939Illumina RNA PCR Primer (100% over 45bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA315800.22252352386504898Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT304230.21437090457714966Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT303300.21371559464303155Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC299550.21107321587642633No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG296740.2090931933873168Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT283140.1995101663937618No Hit
TGACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACT279710.1970932706152402No Hit
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT271550.19134345441910722Illumina RNA PCR Primer (100% over 31bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT266120.1875172899650628Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT265050.18676333122365812Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGTCG260290.18340927177591387No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCA260220.18335994737227057No Hit
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA257060.18113330286494456Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC245620.17307228604095418Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG244880.17255085663101077Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT243320.171451627064103Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGGGAGAGAGAACCCCCCCTCACCTCGACCACGGCCAGTCCGGATCGT242260.1707047146660759No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT239260.16859081165279172No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT238760.168238494483911No Hit
CGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC233600.1646025813010622Illumina RNA PCR Primer (100% over 32bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG220030.15504069333764006Illumina Small RNA Adapter 1 (100% over 26bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG218080.15366665637900534Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA217760.1534411733909217Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT202700.14282938026423506Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG197790.1393696256658266No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG195800.13796740333368143Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC192990.1359873808445719Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT190060.13392280223493103Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG189700.13366913387333693Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAGTCCGGGCCGGATCCGATCGTCGGACTGTAGAACTCTGAACGTGT184320.1298782011361806Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTC182280.12844074708714737No Hit
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT178980.12611545377253475Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA176600.12443842404866261Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA172800.1217608135651693Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC167190.11780781493032787Illumina RNA PCR Primer (100% over 32bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG164750.11608850714619005Illumina Small RNA Adapter 1 (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC154390.108788495406982No Hit
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA153990.10850664167187743Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT153140.10790770248478025Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCCGGTCCGACCGCGACCTCGGAAGATCGTCGGACTGTAGAACTCTGAA152990.10780200733411605Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC151080.10645615574899178No Hit
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA148760.10482140408538533Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGGGAGAGAGAACCCCCCCTCACCTCGACCACGGATCGTCGGACTGTAGA148430.10458887475392409No Hit
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG147260.10376445257874324Illumina Small RNA Adapter 1 (100% over 26bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA145980.10286252062640866Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAACTC145310.10239041562010852Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCAGTCCGGGCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC143970.10144620560750825Illumina RNA PCR Primer (100% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA142340.10029765163695717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)