FastQCFastQC Report
Wed 30 Apr 2025
1G4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14191758
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC11195917.889022628486196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA3902502.7498355031138497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC3386402.386173721395193Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2275991.6037407064015605No Hit
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1798611.2673623662410252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1635231.1522392081375683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1563241.1015125821621254No Hit
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATA1512611.0658369456412657TruSeq Adapter, Index 7 (97% over 35bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATAT1236160.8710407829671277TruSeq Adapter, Index 7 (97% over 35bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1154240.8133171380177143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT989160.6969961015400629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT918710.6473546124447724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA914070.6440851091175597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC901070.6349248627266615Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC885990.6242989769132197Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG859480.605619120619165Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT693470.48864277420739555Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA692600.4880297423335431Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA686190.4835130362284926Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA620780.43742290419551966No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC599720.4225833050422647No Hit
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC554690.390853620812869Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG456690.32179945571225216No Hit
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA453680.3196785063555903Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC430090.30305618232779896Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG416320.29335336749682456No Hit
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC390120.27489194784747595Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA380940.26842340462682635Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG365980.25788207493391585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG347640.24495908117937185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTCCGAGG347040.24453630057671502No Hit
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA340710.24007596521868538Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC339620.23930791379052546No Hit
TTCCGAGGTCGCGGTCGGACCGAGATCGGAAGAGCACACGTCTGAACTCC337560.237856367054737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC317010.2233761314137403No Hit
GACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCTG297070.20932572271877806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA294640.20761346127801786Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA289810.20421007742663033No Hit
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC288710.20343497965509277Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA284860.20072213745471137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC275780.1943240576678379No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC272110.19173804964825358No Hit
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGA258350.18204228116065677No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG257800.18165473227488801Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA255370.1799424708341278TruSeq Adapter, Index 7 (100% over 35bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC251120.17694777489864186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT242420.17081745616011773Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTGGCCGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTTTAGATCG241080.16987324614751745No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA237140.16709698685673757No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT232840.16406705920436354Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC232510.16383452987290228Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC230120.16215045380565254Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC216260.1523842218842796TruSeq Adapter, Index 7 (97% over 35bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC210470.1483043890686411Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC208570.14696558382689445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC205590.14486577350036547Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC197370.13907367924396682No Hit
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC193310.13621286383265555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA189540.1335563923792951Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCAGATCGG183410.1292369838888177No Hit
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT181960.1282152640990637Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC180860.12744016632752617Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC176400.12429749718111034Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG172360.1214507744565543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC169020.11909729576843123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT163740.11537682646505105Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT161310.11366456502429084Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC158060.11137450342656632Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC156550.1103105055765466TruSeq Adapter, Index 7 (97% over 35bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT151850.10699872418906806No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG151290.10660412895992168No Hit
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC150670.1061672556705096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGTCGAGGTGAGGGGGGGTTCTCTCTCCCTAGATCGGAAGAGCACAC146510.1032359768254222No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGATCGGAAGAGCACACGTCTGAAC146130.10296821577707287Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCC143970.10144620560750825Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA142510.10041743947437662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC142020.10007216864887353No Hit
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC142010.1000651223054959Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)