FastQCFastQC Report
Wed 30 Apr 2025
1G2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19974557
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA3842311.923602110424777Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG3393611.6989663400294686Illumina Small RNA Adapter 1 (100% over 26bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT2705901.3546733476992756Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG2472091.2376194375675016Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT2030371.0164781126309834Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1987350.9949407138290977Illumina RNA PCR Primer (100% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1806610.9044556031956053Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT1455920.7288872539200744Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1277540.6395836463356859Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1277460.6395435953848689Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA1250370.6259813421644345Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCAACGACCGGCAGCAGTGATCGTCGGACTGTAGAACTCTGAACGTG957090.47915455646901206Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA934840.4680153857730111Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT908160.4546583936755143Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC830520.41578894590753623Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG828060.41455737916991103Illumina Small RNA Adapter 1 (100% over 26bp)
ACCTACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAG826940.41399666585847184No Hit
GGCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGA787150.3940763241958257Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC751230.37609344727895594Illumina RNA PCR Primer (100% over 30bp)
AGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTCTGA738540.36974036520559633Illumina DpnII expression Sequencing Primer (100% over 24bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT734100.36751753743524823Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT732160.366546301877934Illumina RNA PCR Primer (100% over 44bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT681800.34133422833858096Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG661880.3313615415851275Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA620660.310725289176626Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGA619420.31010449943896123Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTC557910.2793103246294774Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATTACTTCGGTTCCAGAAACCAACAAAATAGAACGATCGTCGGACTGTAG505270.2529567989918375No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGATCGTCGGACTGTAGAACTCT491490.24605802271359511Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG482030.24132199777947516Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAACGTG479340.2399752845582508Illumina DpnII expression Sequencing Primer (96% over 29bp)
TATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAACGTGT474610.2376072720911908Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGTCGGCGCCAGTCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT471350.2359751958453947Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA471270.23593514489457765Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATTCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA465810.2332016675013118Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG461700.23114404990308418Illumina RNA PCR Primer (100% over 33bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC456520.22855075083767817Illumina RNA PCR Primer (100% over 32bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC418770.2096517084208676Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCTCTATTCTTGTTCGCCTAACCGTGATCGTCGGACTGTAGAACTCTG417910.209221160699584Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA414930.2077292627816477Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA411160.20584186172439267Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC402080.2012960788066539Illumina RNA PCR Primer (100% over 30bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG400880.20069531454439765Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCGATCGTCGGAC400100.2003048177739311No Hit
GCCAATCCACGGCCGATTCCGATCGTCGGACTGTAGAACTCTGAACGTGT398540.19952382423299803Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGGTCCGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT373110.1867926282420181Illumina RNA PCR Primer (100% over 31bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG368260.18436453934873248Illumina RNA PCR Primer (100% over 34bp)
TGATCAAGTTTGAACAACTTCCCGGCGATCGTCGGACTGTAGAACTCTGA357670.17906279473432127Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGGGCCATCCCGAAATCCAACTACGGATCGTCGGACTGTAGAACTCTGAA346260.17335052787403496Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACGTGT323790.16210121706328706Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA310190.15529255542438314Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCCCAACGACCGGCAGCAGTCGATCGTCGGACTGTAGAACTCTGAACGT304110.1522486831622849Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCTCTTGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC302200.15129246671152707Illumina RNA PCR Primer (100% over 32bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA301500.1509420208918776Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACT298710.14954524398213187No Hit
CGGCAGCAGTCGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC297980.149179779055926Illumina RNA PCR Primer (100% over 30bp)
TGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAG294890.1476328110806162Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG283170.14176534678591368Illumina Small RNA Adapter 1 (100% over 26bp)
AGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC280590.14047370362206282Illumina RNA PCR Primer (100% over 30bp)
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT275550.13795049372058665Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAACGTGT270820.13558248125352668Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAAC267380.13386029036839214Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACGTCTAAGGAAACATCGTTCTATTCCTATCGATCGTCGGACTGTAGAAC264420.13237840518816013No Hit
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG258790.12955981952440798Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCATTCGCAGTTTCACAGTACTAGGATCGTCGGACTGTAGAACTCTGA256610.12846843111464248Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT255730.12802787065565457Illumina RNA PCR Primer (100% over 50bp)
ACTGCAAGCAGTCCACCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA247400.12385756540182592Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCAACGACCGGCAGCAGGATCGTCGGACTGTAGAACTCTGAACGTGT247210.12376244439363537Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAATCCACGGCCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGA246800.12355718327069783Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA235090.11769472534484744Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG227410.11384983406640758Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG224470.11237796162387982No Hit
GCGAGCTGCTCCCACTTATTCTACACCTCGATCGTCGGACTGTAGAACTC221420.11085101912397857Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT213640.10695606415701735Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTG208690.10447791157521041Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA207300.1037820263047636Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCCACGGCCGATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT207100.10368189892772091Illumina RNA PCR Primer (100% over 31bp)
ACCCGATCTACATAGAACGACTACCCCCAGATCGTCGGACTGTAGAACTC201510.10088333873937731Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGAA199790.10002224329681005Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)